BID 抗体 (AA 61-118)
Our Local Distributor
北京 101111
Quick Overview for BID 抗体 (AA 61-118) (ABIN5540048)
抗原
See all BID 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 61-118
-
特异性
- This antibody reacts with BID.
-
交叉反应 （详细）
- Does not work with Mouse (WR19L, L5178Y, NIH/3T3) and Rat (Rat-1 BHK).
-
纯化方法
- Protein A agarose
-
免疫原
- Recombinant human BID (61-118 aa)
-
亚型
- IgG1
-
-
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibody
BID 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) antibodyBID 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3C5 unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 68-103) antibodyBID 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB0957-RB0958 unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-196) antibodyBID 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-130) antibodyBID 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibodyBID 适用: 人, 小鼠, 大鼠 WB, IHC, ICC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 68-103) antibodyBID 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibodyBID 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 1-195) antibodyBID 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F3-1A3 unconjugated
anti-BH3 Interacting Domain Death Agonist (BID) (AA 39-68) antibodyBID 适用: 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Western blot: 1-5 μg/mL for chemiluminescence detection system. For details see protocol below.
-
实验流程
- SDS-PAGE & Western Blotting 1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mM Tris-HCl, pH 7.2, 250 mM NaCl, 0.1 % NP-40, 2 mM EDTA, 10 % glycerol) containing appropriate protease inhibitors. Incubate it at 4 o C with rotating for 30 minutes, then sonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4 o C and transfer the supernatant to another tube. Measure the protein concentration of the supernatant and add the cold Lysis buffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli's sample buffer. 4) Boil the samples for 2 minutes and centrifuge. Load 10 μ L of the sample per lane in a 1 mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm 2 for 1 hour in a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20 % MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature, or overnight at 4 o C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7.2 containing 1 % skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (The concentration of antibody will depend on condition.) 8) Wash the membrane with PBS-T [0.05 % Tween-20 in PBS] (5 minutes x 6 times). 9) Incubate the membrane with the 1:10,000 HRP-conjugated anti-mouse IgG diluted with 1 % skimmed milk (in PBS, pH 7.2) for 1 hour at room temperature. 10) Wash the membrane with PBS-T (5 minutes x 6 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriate chemilumi nescence reagent for 1 minute. Remove extra reagent from the membrane by dabbing with paper towel, and seal it in plastic wrap. 12) Expose to an X-ray film in a dark room for 5 minutes. Develop the film as usual. The condition for exposure and development may vary. (Positive controls for Western blotting ZR-75-1, U937, HL-60)
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- PBS containing 50 % glycerol, pH 7.2. No preservative is contained.
-
储存液
- Azide free
-
储存条件
- -20 °C
-
储存方法
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
-
-
- BID (BH3 Interacting Domain Death Agonist (BID))
-
别名
- bid
-
背景
- Apoptosis is a major form of cell death characterized by several morphological features that include chromatin condensation and fragmentation, cell membrane blebbing, and formation of apoptotic bodies. BID is an apoptosis promoting protein, which possesses only BH3 domain that is a common structure of Bcl-2 family members. Once full length BID (p22) is cleaved with caspases, cleaved BID (p 15) induces a conformational change and oligomerization of a protein named BAK on mitochondrial membrane. It is thought that oligomerized BAK contribute to forming pore s that release cytochrome c into cytosol.
-
UniProt
- P55957
-
途径
- Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
抗原
-
