Keratin Acidic (AE1) 抗体 (FITC)
Quick Overview for Keratin Acidic (AE1) 抗体 (FITC) (ABIN5540046)
抗原
See all Keratin Acidic (AE1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.
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纯化方法
- Protein A Chromatography
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免疫原
- Human epithelial keratin.
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亚型
- IgG1
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应用备注
- Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or it can also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL (1/200-1/1,000) (Not tested in our lab).
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限制
- 仅限研究用
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状态
- Liquid
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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有效期
- 12 months
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- Keratin Acidic (AE1)
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别名
- acidic cytokeratin
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背景
- Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.
抗原
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