CD3 epsilon 抗体 (Internal Region)
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- 抗原 See all CD3 epsilon (CD3E) 抗体
- CD3 epsilon (CD3E)
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抗原表位
- Internal Region
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适用
- 人
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This CD3 epsilon antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 原理
- CD3E (cytoplasmic)
- 序列
- RKGQRDLYSG LNQR
- 特异性
- This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.
- 交叉反应
- 人, Pig, 大鼠
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 亚型
- IgG
- Top Product
- Discover our top product CD3E Primary Antibody
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- 应用备注
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Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB
Peptide ELISA: antibody detection limit dilution 1:128000.
- 说明
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Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- CD3 epsilon (CD3E)
- 别名
- CD3E (CD3E 产品)
- 背景
- CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro
- 基因ID
- 916, 315609
- NCBI登录号
- NP_000724
- 途径
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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