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CD3 epsilon 抗体 (Internal Region)

Verified CD3E 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
产品编号 ABIN5539697
发货至: 中国
  • 抗原 See all CD3 epsilon (CD3E) 抗体
    CD3 epsilon (CD3E)
    抗原表位
    • 24
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    • 16
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    • 12
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    • 4
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    • 1
    Internal Region
    适用
    • 232
    • 163
    • 51
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    宿主
    • 133
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    • 37
    • 23
    • 20
    • 8
    • 1
    • 1
    山羊
    克隆类型
    • 236
    • 111
    • 6
    多克隆
    标记
    • 137
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    • 1
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    • 1
    This CD3 epsilon antibody is un-conjugated
    应用范围
    • 255
    • 142
    • 103
    • 78
    • 75
    • 72
    • 27
    • 25
    • 23
    • 22
    • 14
    • 14
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    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    原理
    CD3E (cytoplasmic)
    序列
    RKGQRDLYSG LNQR
    特异性
    This antibody is designed to recognize the cytoplasmic domain (aa153-207) of the protein.
    交叉反应
    人, Pig, 大鼠
    纯化方法
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    质量等级
    Verified
    亚型
    IgG
    Top Product
    Discover our top product CD3E Primary Antibody
  • 应用备注

    Western Blot: Approx 22 kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 23.1 kDa according to NP_000724.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. This antibody has been successfully used in WB

    Peptide ELISA: antibody detection limit dilution 1:128000.

    说明

    Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/mL
    缓冲液
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Minimize freezing and thawing.
    储存条件
    -20 °C
    储存方法
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原
    CD3 epsilon (CD3E)
    别名
    CD3E (CD3E 产品)
    背景
    CD3E, CD3e molecule, epsilon (CD3-TCR complex), IMD18, T3E, TCRE, CD3-epsilon, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycopro
    基因ID
    916, 315609
    NCBI登录号
    NP_000724
    途径
    TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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