VEGFA 抗体 (AA 288-299)
Quick Overview for VEGFA 抗体 (AA 288-299) (ABIN5539606)
抗原
See all VEGFA 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- AA 288-299
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原理
- VEGFA (aa288-299)
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序列
- RIKPHQGQHI GE
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特异性
- This antibody is expected to recognize all reported isoforms.
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交叉反应
- 犬, 人, Pig
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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亚型
- IgG
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应用备注
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Immunohistochemistry: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 5 μg/mL.
Western Blot: Approx 45 kDa band observed in lysates of cell line HEK293 (calculated MW of 45.5 kDa according to NP_001020537.2). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat
Peptide ELISA: antibody detection limit dilution 1:128000.
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说明
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Immunofluorescence: Testing has shown that this antibody stains mitochondria in Mouse NIH3T3 cells at 10μg/ml.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGFA
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背景
- VEGFA, vascular endothelial growth factor A, MGC70609, MVCD1, VEGF, VPF, OTTHUMP00000016487, OTTHUMP00000016488, OTTHUMP00000165985, OTTHUMP00000165986, OTTHUMP00000165987, OTTHUMP00000224107, OTTHUMP00000224108, OTTHUMP00000224109, OTTHUMP00000224153, OT
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基因ID
- 7422
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NCBI登录号
- NP_001020537, NP_003367, NP_001020538, NP_001020539
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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