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EGF 抗体 (Internal Region)

This 山羊 多克隆 antibody specifically detects EGF in WB, IHC 和 ELISA. It exhibits reactivity toward 人.
产品编号 ABIN5539508
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for EGF 抗体 (Internal Region) (ABIN5539508)

抗原

See all EGF 抗体
EGF (Epidermal Growth Factor (EGF))

适用

  • 158
  • 90
  • 49
  • 12
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  • 4
  • 1
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宿主

  • 224
  • 35
  • 9
  • 2
  • 2
  • 1
山羊

克隆类型

  • 216
  • 56
多克隆

标记

  • 124
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This EGF antibody is un-conjugated

应用范围

  • 207
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Western Blotting (WB), Immunohistochemistry (IHC), ELISA

质量等级

Verified
  • 抗原表位

    • 45
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    • 1
    Internal Region

    原理

    Pro-EGF

    序列

    SRQERVCNIE KNVS

    特异性

    This antibody is expected to recognize all reported isoforms (NP_001954.2, NP_001171601.1, NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.

    交叉反应

    犬, 人

    纯化方法

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    亚型

    IgG
  • 应用备注

    Immunohistochemistry: Paraffin embedded Human Skin. Recommended concentration: 5 μg/mL.

    Western Blot: Approx 160 kDa double band observed in Human Prostate lysates (calculated MW of 134 kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3 μg/mL. Primary incubation was 1

    Peptide ELISA: antibody detection limit dilution 1:128000.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.5 mg/mL

    缓冲液

    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Minimize freezing and thawing.

    储存条件

    -20 °C

    储存方法

    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • 抗原

    EGF (Epidermal Growth Factor (EGF))

    别名

    EGF

    背景

    EGF, epidermal growth factor, HOMG4, URG, OTTHUMP00000162922, OTTHUMP00000219721, OTTHUMP00000219722, beta-urogastrone, pro-epidermal growth factor

    基因ID

    1950

    NCBI登录号

    NP_001954, NP_001171601, NP_001171602

    途径

    NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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