This antibody is purified through a protein A column, followed by peptide affinity purification.
免疫原
This EGFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-287 amino acids from the N-terminal region of human EGFR.
EGF
适用: 人
ELISA, IF (p), IF (cc), IHC (p), FACS, IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
For IHC-P starting dilution is: 1:25
For WB starting dilution is: 1:1000
限制
仅限研究用
状态
Liquid
浓度
0.5 mg/mL
缓冲液
Supplied in PBS with 0.09 % (W/V) sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C,-20 °C
储存方法
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
抗原
EGF
(Epidermal Growth Factor (EGF))
别名
EGF
背景
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.