MED14 抗体 (AA 181-375)
Quick Overview for MED14 抗体 (AA 181-375) (ABIN5519034)
抗原
See all MED14 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 181-375
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原理
- Anti-MED14 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-MED14 Antibody Picoband® (ABIN5519034). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human MED14 recombinant protein (Position: D181-D375).
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亚型
- IgG
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anti-Mediator Complex Subunit 14 (MED14) (Internal Region) antibody
MED14 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (AA 586-615) antibodyMED14 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB20179 unconjugated
anti-Mediator Complex Subunit 14 (MED14) antibodyMED14 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (AA 575-624) antibodyMED14 适用: 人, 小鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (AA 701-750) antibodyMED14 适用: 人, 小鼠 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mediator Complex Subunit 14 (MED14) (Middle Region) antibodyMED14 适用: 人, 小鼠, 兔 WB, ChIP 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL
ELISA, 0.1-0.5 μg/mL
1. Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). "The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1". Nature. 397 (6718): 446-50. 2. Yoshikawa H, Fujiyama A, Nakai K, Inazawa J, Matsubara K (Aug 1998). "Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method". Genomics. 49 (2): 237-46. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- MED14 (Mediator Complex Subunit 14 (MED14))
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别名
- MED14
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背景
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Synonyms: Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRSP complex subunit 2, Mediator complex subunit 14, RGR1 homolog, hRGR1, Thyroid hormone receptor-associated protein complex 170 kDa component, Trap170, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, DRIP150, MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170,
Tissue Specificity: Ubiquitous.
Background: Mediator of RNA polymerase II transcription subunit 14 is an enzyme that in humans is encoded by the MED14 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.
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分子量
- 160 kDa
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基因ID
- 9282
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UniProt
- O60244
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
抗原
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