ATG3 抗体 (AA 1-244)
Quick Overview for ATG3 抗体 (AA 1-244) (ABIN5518892)
抗原
See all ATG3 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-244
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原理
- Anti-Apg3/ATG3 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins.
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产品特性
- Anti-Apg3/ATG3 Antibody Picoband® (ABIN5518892). Tested in WB applications. This antibody reacts with Human, Monkey, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human < a href="https://www.bosterbio.com/anti-apg3-atg3-rabbit-monoclonal-antibody-m01768-boster.html" style="color:#EA8D28">Apg3 recombinant protein (Position: M1-T244). Human Apg3 shares 96.7% and 96.3% amino acid (aa) sequence identity with mouse and rat Apg3, respectively.
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亚型
- IgG
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anti-ATG3 Autophagy Related 3 (ATG3) (AA 1-100) antibody
ATG3 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 2C10A12 unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) (AA 1-100) antibodyATG3 适用: 人, 猴 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 7A1D1 unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) antibodyATG3 适用: 人, 小鼠 WB, IHC (p) 宿主: 小鼠 Monoclonal 7C2 unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) (AA 1-314) antibodyATG3 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) antibodyATG3 适用: 人, 大鼠, 犬, 猴 WB, IF, FACS 宿主: 小鼠 Monoclonal 3C6 unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) antibodyATG3 适用: 人, 大鼠, 犬, 猴 WB, IF, FACS 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) (Middle Region) antibodyATG3 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) (AA 1-100) antibodyATG3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G3 unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) (AA 51-150) antibodyATG3 适用: 小鼠, 大鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-ATG3 Autophagy Related 3 (ATG3) (N-Term) antibodyATG3 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse
1. Tanida, I., Tanida-Miyake, E., Komatsu, M., Ueno, T., Kominami, E. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p. J. Biol. Chem. 277: 13739-13744, 2002. 2. Tsuboyama, K., Koyama-Honda, I., Sakamaki, Y., Koike, M., Morishita, H., Mizushima, N. The ATG conjugation systems are important for degradation of the inner autophagosomal membrane. Science 354: 1036-1041, 2016. -
说明
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We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- ATG3 (ATG3 Autophagy Related 3 (ATG3))
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别名
- ATG3
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背景
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Synonyms: Ubiquitin-like-conjugating enzyme ATG3,6.3.2.-,Autophagy-related protein 3,APG3-like,hApg3,Protein PC3-96,ATG3,APG3, APG3L,
Tissue Specificity: Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta. .
Background: This ATG3 gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. And the protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms.
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分子量
- 36-40 kDa
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基因ID
- 64422
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途径
- Autophagy
抗原
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