Crk 抗体 (pTyr221)
Quick Overview for Crk 抗体 (pTyr221) (ABIN498832)
抗原
See all Crk (CRK) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr221
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特异性
- This antibody detects endogenous levels of p-Crk2 protein only when phosphorylated at Try221.
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) antibody
CRK 适用: 人 ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3G11C1 unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (AA 1-204) antibodyCRK 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (pTyr221) antibodyCRK 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (AA 2-246) antibodyCRK 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (AA 1-204) antibodyCRK 适用: 人 WB, PLA 宿主: 小鼠 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (AA 122-302) antibodyCRK 适用: 人, 小鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (Center) antibodyCRK 适用: 人, 小鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (pTyr221) antibodyCRK 适用: 人 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) (AA 40-196) antibodyCRK 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK) antibodyCRK 适用: 人, 小鼠, 大鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))
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别名
- CRK
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背景
- The Crk family of adapter proteins including Crk II, Crk I and Crk L consist mostly of SH2 and SH3 domains. Through the interactions between SH2 domain and phosphotyrosine residues and/or between SH3 domain and proline- rich motifs, they are involved in a variety of signaling cascades. Crk I and Crk II are encoded by the same gene, which undergoes alternative splicing to yield these two proteins, but differ in their biological activities. Crk II has less transforming activity than Crk I, although both Crk I and Crk II bind to many tyrosine-phosphorylated proteins that bind to GRB2. In addition, Crk II becomes rapidly tyrosine-phosphorylated in response to stimulation with insulin-like growth factor-I (IGFI) and might be involved in the IGF-I receptor signalling pathway. The gene encoding Crk I and II maps to human chromosome 17p13, a region which demonstrates frequent deletion or loss of heterozygosity in a wide range of human cancers.Synonyms: Adapter molecule crk, Proto-oncogene C-crk, p38
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分子量
- approx. 42 kDa
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基因ID
- 1398
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NCBI登录号
- NP_005197
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UniProt
- P46108
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途径
- Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
抗原
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