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EIF2AK2 抗体 (pThr446)

Name: EIF2AK2 抗体 (pThr446)
SKU: ABIN498762
Availability: OutOfStock

EIF2AK2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

Western blot (WB) analyzes of p-PKR (pThr446) antibody in extracts from NIH-3T3 IFN cells. (EIF2AK2 抗体 (pThr446))

1 image

产品编号 ABIN498762

发货至: 中国

Datasheet as PDF

抗原 See all EIF2AK2 抗体

EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))
抗原表位
45

34

10

6

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pThr446
适用
122

71

47

2

2

1

1

1

1
人
宿主
116

10
兔
Compatible Secondaries
克隆类型
105

21
多克隆
标记
73

5

4

4

4

4

4

4

4

4

3

3

3

3

3

1
This EIF2AK2 antibody is un-conjugated
应用范围
105

46

41

40

37

27

19

16

9

7

5

4

2

1
Western Blotting (WB)

特异性

This antibody detects endogenous levels of PKR protein only when phosphorylated at Thr446.

交叉反应（详细）

Species reactivity (tested):Human.

纯化方法

Affinity chromatography

纯度

> 95 % by SDS-PAGE

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EIF2AK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D11 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibody
EIF2AK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1B9 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 329-551) antibody
EIF2AK2 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 6H3A10 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 224-502) antibody
EIF2AK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 1-100) antibody
EIF2AK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2C2 unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 11-42) antibody
EIF2AK2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (AA 519-550) antibody
EIF2AK2 适用: 人, 小鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr446) antibody
EIF2AK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr451) antibody
EIF2AK2 适用: 人, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2) (pThr446), (pThr451) antibody
EIF2AK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1,0 mg/mL

缓冲液

Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

别名

EIF2AK2 / PKR (EIF2AK2 产品)

别名

PKR antibody, pkr antibody, EIF2AK2 antibody, DKFZp469K1934 antibody, EIF2AK1 antibody, PRKR antibody, 2310047A08Rik antibody, 4732414G15Rik antibody, AI467567 antibody, AI747578 antibody, Pkr antibody, Prkr antibody, Tik antibody, eukaryotic translation initiation factor 2 alpha kinase 2 antibody, eukaryotic translation initiation factor 2-alpha kinase 2 antibody, EIF2AK2 antibody, eif2ak2 antibody, Eif2ak2 antibody

背景

An interferon-inducible, RNA-dependent protein Serine/Threonine kinase, PKR has various designations. Mouse PKR is known as DAI, dsJ, PI kinase, p65, p67 or TIK, whereas human PKR is known as p68 or p69. PKR phosphorylates its substrate, a subunit of protein synthesis initiation factor eIF-2, on Ser 51 to inhibit translation. PKR contains two dsRNA binding motifs required for its activation by dsRNA. Three kinds of regulation of PKR enzymatic activity occur, and these include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation, and post-translational regulation by RNA mediated autophosphorylation. Human PKR contains at least 15 autophosphorylation sites, but only Thr 446 and Thr 451 in the activation loop are critical for its kinase activity. Thr 446 is the in vivo autophosphorylation site of PKR. Mutation of Threonine to alanine at position 446 substantially reduces PKR function, and mutant kinase containing Ala 451 is completely inactive.Synonyms: Eukaryotic translation initiation factor 2-alpha kinase 2, Interferon-induced double-stranded RNA-activated protein kinase, PRKR, Protein kinase RNA-activated, eIF-2A protein kinase 2, p68 kinase

分子量

Predicted band size: 62 kDaObserved band size: 68 kDa

基因ID

5610

NCBI登录号

NP_001129123

UniProt

P19525

途径

DNA Damage Repair, ER-Nucleus Signaling, Hepatitis C