BNIP3 抗体
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Quick Overview for BNIP3 抗体 (ABIN498556)
抗原
See all BNIP3 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of BNIP-3 protein. (region surrounding Glu96)
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 50-155) antibody
BNIP3 适用: 人 IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6A5F7 unconjugated
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 1-164) antibodyBNIP3 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 50-155) antibodyBNIP3 适用: 人 ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 4E11F4 unconjugated
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 152-187) antibodyBNIP3 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 3-134) antibodyBNIP3 适用: 大鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 1-164) antibodyBNIP3 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) antibodyKO Validated BNIP3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 25GB4120 unconjugated Recombinant Antibody
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) antibodyKD Validated BNIP3 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB13585 unconjugated Recombinant Antibody
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 112-124) antibodyBNIP3 适用: 人 WB, IHC, ELISA, IHC (p), IP 宿主: 小鼠 Monoclonal unconjugated
anti-BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3) (AA 215-252) antibodyBNIP3 适用: 人, 小鼠 IF, IHC (p) 宿主: 兔 Polyclonal RB3385 unconjugated
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应用备注
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Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
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别名
- NIP3
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背景
- BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic, mitochondrial protein classified in the Bcl 2 family based on limited sequence homology to the Bcl 2 homology 3 (BH3) domain (amino acids 110-118) and C-terminal TM domain. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl 2, Bcl XL, CED9 and the adenovirus E1B 19K protein. Typically the BH3 domain of pro-apoptotic Bcl-2 homologues mediates Bcl 2/Bcl XL heterodimerization and confers pro-apoptotic activity. BNIP3 represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and nonmitochondrial sites by selective Bcl 2/Bcl XL interactions. The N-terminus (residues 1-49) and the C-terminus TM domain of BNIP3 are critical for Bcl 2 heterodimerization, and either region is sufficient for Bcl XL interaction. The TM domain of BNIP3 is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting. BNIP3 contains PEST sequences suggesting that the protein may be susceptible to rapid degradation by proteases. PEST sequences commonly contain high local concentrations of amino acids P, E, S, T, and D flanked by charged amino acids and these are abundantly present in NIP3. Thus, the posttranslational control of BNIP3 expression through rapid protein degradation may constitute a mechanism for regulating the intracellular levels of a potentially lethal protein.Synonyms: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP3
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分子量
- approx. 22 kDa
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基因ID
- 664
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NCBI登录号
- NP_004043
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UniProt
- Q12983
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途径
- Autophagy, Brown Fat Cell Differentiation
抗原
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