PRKAG1 抗体
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北京 101111
Quick Overview for PRKAG1 抗体 (ABIN498427)
抗原
See all PRKAG1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of AMPKgamma1/2/3 protein. (region surrounding Lys78)
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交叉反应 (详细)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography
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纯度
- > 95 % by SDS-PAGE
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应用备注
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Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1,0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- PRKAG1 (Protein Kinase, AMP-Activated, gamma 1 Non-Catalytic Subunit (PRKAG1))
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别名
- AMPK gamma-1 Chain / AMPKg
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背景
- The 5-AMP activated protein kinase (AMPK), a member of the SNF1 (sucrose non fermentor) kinase family, is a heterotrimeric protein comprise of alpha (63 kDa), beta (30 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit, while beta and gamma are non catalytic subunits (although they have been found to interact with the active subunit in liver). AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase. AMPK is activated by AMP and can be also regulated by treatment with purified protein phosphatase in vitro.Synonyms: 5'-AMP-activated protein kinase subunit gamma-1/2/3, AMPK gamma-1, AMPK gamma-2, AMPK gamma-3, AMPKG3, AMPKg, H91620p, PRKAG1, PRKAG2, PRKAG3
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分子量
- approx. 40,42 kDa
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基因ID
- 5571
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NCBI登录号
- NP_002724
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UniProt
- P54619
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途径
- AMPK Signaling, Regulation of Carbohydrate Metabolic Process, Warburg Effect
抗原
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