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CK1 epsilon 抗体

The 兔 多克隆 anti-CK1 epsilon antibody is suitable to detect CK1 epsilon in samples from 人 和 小鼠. It has been validated for WB, IF 和 IHC (p).
产品编号 ABIN498317
发货至: 中国
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Quick Overview for CK1 epsilon 抗体 (ABIN498317)

抗原

See all CK1 epsilon (CSNK1E) 抗体
CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This CK1 epsilon antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 特异性

    This antibody detects endogenous levels of Casein Kinase Iε protein. (region surrounding Pro301)

    交叉反应 (详细)

    Species reactivity (expected):Mouse.
    Species reactivity (tested):Human.

    纯化方法

    Affinity chromatography using epitope-specific immunogen

    纯度

    > 95 % (by SDS-PAGE)
  • 应用备注

    Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
  • 抗原

    CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

    别名

    Casein Kinase I epsilon

    背景

    Casein kinase I (also designated CKI) and casein kinase II (also designated CKII) compose a family of serine/ threonine protein kinases which are present in all eukaryotes examined to date. CKI family members, which include CKIα, γ, ε and δ, have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. CKII is usually expressed as a tetrameric complex consisting of either an α2β2 or an αα'β2 structure. The α catalytic subunit is stimulated by the β regulatory subunit, which undergoes autophosphorylation. CKII activity is high in the cytosol and nucleus of proliferating and differentiating cells. CKII is known to phosphorylate more than 100 different substrates including nuclear oncoproteins, transcription factors and enzymes involved in DNA metabolism.Synonyms: CKI-epsilon, CKIe, CSNK1E

    分子量

    approx. 47 kDa

    基因ID

    1454

    NCBI登录号

    NP_001885

    UniProt

    P49674

    途径

    Hedgehog Signaling, M Phase
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