CDC25A 抗体
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北京 101111
Quick Overview for CDC25A 抗体 (ABIN498254)
抗原
See all CDC25A 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Cdc25A protein.
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交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. -
纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibody
CDC25A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB07634 unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 235-484) antibodyCDC25A 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer75) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibodyCDC25A 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer178) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pThr507) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (pSer124) antibodyCDC25A 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 1-524) antibodyCDC25A 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (AA 480-514), (pThr507) antibodyCDC25A 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7856 unconjugated
anti-Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A) (Ser124) antibodyCDC25A 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB59086 unconjugated
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应用备注
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Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))
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别名
- CDC25A
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背景
- The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40 % identity in their most conserved carboxy terminal sequences.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1
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分子量
- approx. 59 kDa
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基因ID
- 993
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NCBI登录号
- NP_001780
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UniProt
- P30304
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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