RASGRF1 抗体
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Quick Overview for RASGRF1 抗体 (ABIN498226)
抗原
See all RASGRF1 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- This antibody detects endogenous levels of Ras-GRF1 protein.
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交叉反应 （详细）
- Species reactivity (tested):Mouse and Rat.
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纯化方法
- Affinity chromatography using epitope-specific immunogen
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纯度
- > 95 % (by SDS-PAGE)
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anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (Middle Region) antibody
RASGRF1 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 1-190) antibodyRASGRF1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 907-936) antibodyRASGRF1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB14017 unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibodyRASGRF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibodyRASGRF1 适用: 人 ELISA, WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (pSer916) antibodyRASGRF1 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 467-516) antibodyRASGRF1 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼, 猴, Pig, Bat, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (AA 107-156) antibodyRASGRF1 适用: 小鼠, 人, Cow, 犬, 豚鼠, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1) (N-Term) antibodyRASGRF1 适用: 小鼠, 大鼠, 人, Cow, 犬, 豚鼠, 马, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- RASGRF1 (Ras Protein-Specific Guanine Nucleotide-Releasing Factor 1 (RASGRF1))
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别名
- RASGRF1
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背景
- A critical step in signal transduction responses to stimulation of cell surface receptors by their ligands involves the accumulation of Ras proteins in their active GTP-bound state. To reach their active GTP-bound state, Ras proteins must first release bound GDP, a rate limiting step mediated by a guanine nucleotide releasing factor (GRF). The mammalian Ras p21 GRF protein has been designated Ras-GRF1 p140. Ras-GRF1 accelerates release of GDP from H- and N-Ras p21 protein in vitro, but not from the related Ral A or Cdc42Hs GTP-binding proteins. Of interest, a region mapping within the amino terminal domain of Ras-GRF1 is similar to both the human breakpoint cluster protein, Bcr, and the Dbl proto-oncogene product, a guanine nucleotide-releasing factor for Cdc42Hs. Ras-GRF2 p135 has also been identified. Ras-GRF2 p135 is highly homologous to Ras-GRF1 p140 except in the region between the REM and CDC25 domains and appears to function similarly to Ras-GRF1 p140.Synonyms: CDC25, GNRP, GRF1, Guanine nucleotide-releasing protein, Ras-GRF1, Ras-specific guanine nucleotide-releasing factor 1, Ras-specific nucleotide exchange factor CDC25
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基因ID
- 19417
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NCBI登录号
- NP_035375
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UniProt
- P27671
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途径
- RTK signaling, Cell Division Cycle, M Phase
抗原
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