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RPA2 抗体 (AA 115-129)

This anti-RPA2 antibody is a 兔 多克隆 antibody detecting RPA2 in WB, IF 和 IHC (p). Suitable for 人 和 小鼠.
产品编号 ABIN498167
发货至: 中国
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Quick Overview for RPA2 抗体 (AA 115-129) (ABIN498167)

抗原

See all RPA2 抗体
RPA2 (Replication Protein A2, 32kDa (RPA2))

适用

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人, 小鼠

宿主

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克隆类型

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多克隆

标记

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This RPA2 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
  • 抗原表位

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    AA 115-129

    特异性

    This antibody detects endogenous levels of RFA2 protein.

    交叉反应 (详细)

    Species reactivity (expected):Mouse.
    Species reactivity (tested):Human.

    纯化方法

    Affinity chromatography using epitope-specific immunogen

    纯度

    > 95 % (by SDS-PAGE)

    免疫原

    synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.
  • 应用备注

    Western blot: 1: 500 - 1: 1000. immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.

    限制

    仅限研究用
  • 浓度

    1.0 mg/mL

    缓冲液

    Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

    储存液

    Sodium azide

    注意事项

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid repeated freezing and thawing.

    储存条件

    4 °C/-20 °C

    储存方法

    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
  • 抗原

    RPA2 (Replication Protein A2, 32kDa (RPA2))

    别名

    RPA2

    背景

    This phospho specific polyclonal antibody is specific for phosphorylated pS122 of yeast RFA2. Reactivity with non-phosphorylated yeast RFA2 is minimal by ELISA. Immunogen: synthetic peptide corresponding to aa 115-129 (of 273) of yeast DNA Replication Factor A (RFA2). The RFA complex consists of a heterotrimer of RFA1, RFA2 and RFA3, 69, 36 and 13 kda chains respectively.Synonyms: REPA2, RF-A protein 2, RFA2, RP-A p32, RP-A p34, RPA32, RPA34, Replication factor A protein 2, Replication protein A, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit

    分子量

    approx. 32 kDa

    基因ID

    855404

    NCBI登录号

    NP_014087

    UniProt

    P26754

    途径

    Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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