RANBP1 抗体 (N-Term)
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Quick Overview for RANBP1 抗体 (N-Term) (ABIN497936)
抗原
See all RANBP1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1-187, N-Term
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特异性
- Recognizes RAN binding protein 1/RANBP1 (1-187).
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交叉反应 (详细)
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Species reactivity (expected):Rat (97 %), Bovine (88 %).
Species reactivity (tested):Human, Mouse. -
纯化方法
- Antigen-Affinity Chromatography.
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免疫原
- Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 187 of Ran BP-1
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应用备注
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Suitable for use in Western blotting, and Immunocytochemistry/Immunofluorescence. Recommended Dilutions: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Positive Controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1.0 mg/mL
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缓冲液
- 0.1 M Tris, 0.1 M Glycine, 10 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.
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储存液
- Thimerosal (Merthiolate)
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注意事项
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Store the antibody undiluted (in aliquots) at -20 °C.
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- RANBP1 (RAN Binding Protein 1 (RANBP1))
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别名
- RANBP1
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背景
- Ran/TC4-binding protein, RanBP1, interacts specifically with GTP-charged RAN. RANBP1 encodes a 23-kD protein that binds to RAN complexed with GTP but not GDP. RANBP1 does not activate GTPase activity of RAN but does markedly increase GTP hydrolysis by the RanGTPase-activating protein (RanGAP1). The RANBP1 cDNA encodes a 201-amino acid protein that is 92 % similar to its mouse homolog. In both mammalian cells and in yeast, RANBP1 acts as a negative regulator of RCC1 by inhibiting RCC1-stimulated guanine nucleotide release from RAN.Synonyms: Ran-binding protein 1, Ran-specific GTPase-activating protein
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基因ID
- 5902, 9606
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UniProt
- P43487
抗原
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