ErbB2/Her2 抗体 (pTyr1248)
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Quick Overview for ErbB2/Her2 抗体 (pTyr1248) (ABIN3029494)
抗原
See all ErbB2/Her2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pTyr1248
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原理
- HER2 Antibody / ErbB2 (pY1248)
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纯化方法
- Antigen affinity purified
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免疫原
- This p-ErbB2 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pY1248 of human HER2/ErbB2.
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亚型
- Ig Fraction
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anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 23-372) antibody
ErbB2/Her2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (C-Term) antibodyErbB2/Her2 适用: 人, 小鼠, 大鼠, 犬, 猴, 马, Pig, Cow, 兔, Hamster, Avian, Cat, 小鸡, 驴, 山羊, 豚鼠, 绵羊 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 22-121) antibodyErbB2/Her2 适用: 人 IF, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 3B1 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 适用: 人 IHC, IF, FACS 宿主: 小鼠 Monoclonal ERBB2-2452 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 311-462) antibodyErbB2/Her2 适用: 人 IHC 宿主: 小鼠 Monoclonal ERBB2-3078 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (AA 1205-1255) antibodyErbB2/Her2 适用: 人, 小鼠 WB, IHC, FACS, IP, ICC 宿主: 兔 Monoclonal BLR241L unconjugated Recombinant Antibody
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (N-Term) antibodyErbB2/Her2 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (Extracellular Domain) antibodyErbB2/Her2 适用: 人 IF, ELISA, FACS 宿主: 小鼠 Monoclonal HRB2-282 unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (C-Term) antibodyErbB2/Her2 适用: 人 IHC, IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2) (pTyr1196) antibodyErbB2/Her2 适用: 人 WB, IF, DB 宿主: 兔 Polyclonal RB40085 unconjugated
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应用备注
- Titration of the Phospho-ErbB2 (pY1248) Antibody / Receptor Activation Marker may be required due to differences in protocols and secondary/substrate sensitivity.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze-thaw cycles
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储存条件
- -20 °C
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储存方法
- Aliquot the p-ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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别名
- Erbb2
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背景
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ErbB2 receptor tyrosine kinase 2 (ERBB2), also known as HER2, is a transmembrane receptor that plays a central role in regulating cell proliferation, survival, and differentiation through activation of intracellular signaling pathways. Phospho-ErbB2 (pY1248) antibody, also referred to as phospho-HER2 antibody and phospho-ERBB2 antibody in the literature, is designed to detect phosphorylation at tyrosine 1248, one of the most widely studied and functionally important sites associated with HER2 activation. Additional HER2 phosphorylation sites can be analyzed using phospho-specific antibodies targeting residues such as < a href=../tds/phospho-erbb2-antibody-py1221-f52419>pY1221 (signaling docking), < a href=../tds/phospho-erbb2-antibody-py1222-f52420>pY1222 (signal propagation), and < a href=../tds/phospho-erbb2-antibody-py1112-f48394>pY1112 (adaptor recruitment).
Phosphorylation at Y1248 occurs following receptor dimerization and activation of the intrinsic tyrosine kinase domain. This modification is closely associated with activation of downstream signaling pathways such as PI3K/AKT and MAPK, which regulate cell growth, survival, and metabolic adaptation. Because of this, phosphorylation at Y1248 is widely used as a direct indicator of active HER2 signaling. Detection of this site allows researchers to distinguish between inactive receptor pools and signaling-competent receptor populations, which is critical when studying pathway dynamics.
HER2 signaling is particularly relevant in cancer biology, where ERBB2 amplification leads to elevated receptor expression and increased signaling output. In breast carcinoma and other HER2-positive tumors, phosphorylation at Y1248 correlates with enhanced pathway activation, increased proliferation, and aggressive tumor behavior. Monitoring this phosphorylation event provides insight into signaling status and therapeutic response, especially in experimental systems evaluating pathway inhibition.
Structurally, Y1248 is located within the intracellular kinase domain of HER2 and functions as part of a coordinated phosphorylation network. Nearby residues such as Y1221 and Y1222 contribute to recruitment of adaptor proteins and early signaling events, while Y1248 reflects overall receptor activation status. This makes Y1248 one of the most reliable and widely used sites for assessing HER2 signaling activity.
Phosphorylation of HER2 is not static and is regulated dynamically in response to cellular conditions, including growth factor availability, receptor dimerization, and cellular stress. Detection of phosphorylated ERBB2 at Y1248 therefore provides a snapshot of signaling activity at a given time, enabling researchers to study pathway activation in response to experimental manipulation or disease progression.
Phospho-specific detection of ERBB2 at Y1248 enables precise analysis of signaling pathways in both normal and pathological contexts. This approach complements total HER2 detection and provides a more complete understanding of receptor function, activation status, and signaling output.
For a full selection of HER2 antibodies for research applications, see our < a href=../her2-antibody.html>HER2 antibody page. -
UniProt
- P04626
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
抗原
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