CD73 抗体 (AA 520-550)
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Quick Overview for CD73 抗体 (AA 520-550) (ABIN3029822)
抗原
See all CD73 (NT5E) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 520-550
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纯化方法
- Purified
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免疫原
- A portion of amino acids 520-550 from the human protein was used as the immunogen for this CD73 antibody.
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亚型
- Ig Fraction
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anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibody
NT5E 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 4G6B10 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 29-500) antibodyNT5E 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 290-550) antibodyNT5E 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal NT5E-2503 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibodyNT5E 适用: 人 WB, IHC, FACS, IF, ELISA, StM, Coat 宿主: 小鼠 Monoclonal NT5E-2545 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 30-250) antibodyNT5E 适用: 人 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 4G6E3 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 520-550), (C-Term) antibodyNT5E 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB02846 unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 48-527) antibodyNT5E 适用: Cow WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) antibody (PE)NT5E 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal AD2 PE
anti-5'-Nucleotidase, Ecto (CD73) (NT5E) (AA 151-250) antibodyNT5E 适用: 人, 小鼠, 大鼠, Cow, 犬, 绵羊 WB, FACS, ELISA, ICC, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Titration of the CD73 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000-2000,IHC (Paraffin): 1:10-1:50
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- In 1X PBS, pH 7.4, with 0.09 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Aliquot the CD73 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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- CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))
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别名
- CD73
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背景
- Ecto-5-prime-nucleotidase/5-prime-ribonucleotide phosphohydrolase/NT5E/CD73 catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70 kD subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5E/CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. It is not known whether the different enzymes are coded by different genes or result from different posttranslational modifications of a single coding sequence.
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UniProt
- P21589
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途径
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
抗原
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