ATG14 抗体
Quick Overview for ATG14 抗体 (ABIN4950253)
抗原
See all ATG14 抗体适用
宿主
克隆类型
标记
应用范围
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纯化方法
- Antigen affinity
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免疫原
- Amino acids RDRERFIDKKERLSRLKSKQEEFQKEVLKAME of human ATG14L were used as the immunogen for the ATG14L antibody.
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亚型
- IgG
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anti-ATG14 Autophagy Related 14 (ATG14) (AA 43-303) antibody
ATG14 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F7C10 unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-492) antibodyATG14 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-310) antibodyATG14 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 70-101), (N-Term) antibodyATG14 适用: 人, 大鼠 WB, IHC, FACS, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-310) antibodyATG14 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 162-190) antibodyATG14 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19671 unconjugated
anti-ATG14 Autophagy Related 14 (ATG14) (AA 55-84), (N-Term) antibodyATG14 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB19669 unconjugated
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应用备注
- Optimal dilution of the ATG14L antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the ATG14L antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- ATG14 (ATG14 Autophagy Related 14 (ATG14))
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别名
- ATG14L
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背景
- ATG14 (also known as beclin-1-associated autophagy-related key regulator (Barkor) or ATG14L), an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29, and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8). ATG14 binds to the SNARE core domain of STX17 through its coiled-coil domain, and stabilizes the STX17-SNAP29 binary t-SNARE complex on autophagosomes.
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UniProt
- Q6ZNE5
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途径
- Autophagy
抗原
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