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ATG14 抗体

The 兔多克隆 anti-ATG14 antibody is suitable to detect ATG14 in samples from 人和大鼠. It has been validated for WB, FACS 和 IHC (p).

Western blot testing of 1) rat brain and 2) human HeLa lysate with ATG14L antibody. (ATG14 抗体)

IHC testing of FFPE rat spleen with ATG14L antibody. (ATG14 抗体)

Flow cytometry testing of human A431 cells with ATG14L antibody at 1ug/10^6 cells (blocked with goat sera) (ATG14 抗体)

Flow cytometry testing of human SiHa cells with ATG14L antibody at 1ug/10^6 cells (blocked with goat sera) (ATG14 抗体)

Flow cytometry testing of human PC-3 cells with ATG14L antibody at 1ug/10^6 cells (blocked with goat sera) (ATG14 抗体)

5 images

产品编号 ABIN4950253

发货至: 中国

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Quick Overview for ATG14 抗体 (ABIN4950253)

抗原

See all ATG14 抗体

ATG14 (ATG14 Autophagy Related 14 (ATG14))

适用

人, 大鼠

宿主

兔

克隆类型

多克隆

标记

20
3
3
2
1
1
1
1
1
1
1
1

This ATG14 antibody is un-conjugated

应用范围

22
20
12
7
7
2
1
1
1

Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product ATG14 Primary Antibody

纯化方法

Antigen affinity

免疫原

Amino acids RDRERFIDKKERLSRLKSKQEEFQKEVLKAME of human ATG14L were used as the immunogen for the ATG14L antibody.

亚型

IgG
anti-ATG14 Autophagy Related 14 (ATG14) (AA 43-303) antibody
ATG14 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1F7C10 unconjugated

anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-492) antibody
ATG14 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-ATG14 Autophagy Related 14 (ATG14) (AA 70-101), (N-Term) antibody
ATG14 适用: 人, 大鼠 WB, IHC, FACS, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-310) antibody
ATG14 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-ATG14 Autophagy Related 14 (ATG14) (AA 55-84), (N-Term) antibody
ATG14 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal RB19669 unconjugated

anti-ATG14 Autophagy Related 14 (ATG14) (AA 162-190) antibody
ATG14 适用: 人 WB, FACS 宿主: 兔 Polyclonal RB19671 unconjugated

anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-492) antibody (FITC)
ATG14 适用: 人宿主: 兔 Polyclonal FITC

anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-492) antibody (HRP)
ATG14 适用: 人 ELISA 宿主: 兔 Polyclonal HRP

anti-ATG14 Autophagy Related 14 (ATG14) (AA 1-492) antibody (Biotin)
ATG14 适用: 人 ELISA 宿主: 兔 Polyclonal Biotin

anti-ATG14 Autophagy Related 14 (ATG14) antibody
ATG14 适用: 人, 小鼠, 大鼠 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal dilution of the ATG14L antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,FACS: 1-3 μg/10^6 cells

限制

仅限研究用
缓冲液

0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

储存条件

-20 °C

储存方法

After reconstitution, the ATG14L antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
抗原

ATG14 (ATG14 Autophagy Related 14 (ATG14))

别名

ATG14L

背景

ATG14 (also known as beclin-1-associated autophagy-related key regulator (Barkor) or ATG14L), an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29, and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8). ATG14 binds to the SNARE core domain of STX17 through its coiled-coil domain, and stabilizes the STX17-SNAP29 binary t-SNARE complex on autophagosomes.

UniProt

Q6ZNE5

途径

Autophagy

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