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Topoisomerase II alpha 抗体 (AA 1352-1493)

This anti-Topoisomerase II alpha antibody is a 小鼠 单克隆 antibody detecting Topoisomerase II alpha in WB, IF, FACS 和 IHC (p). Suitable for 人 和 小鼠.
产品编号 ABIN4949922
发货至: 中国
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Quick Overview for Topoisomerase II alpha 抗体 (AA 1352-1493) (ABIN4949922)

抗原

See all Topoisomerase II alpha (TOP2A) 抗体
Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

适用

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人, 小鼠

宿主

  • 119
  • 38
小鼠

克隆类型

  • 95
  • 62
单克隆

标记

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This Topoisomerase II alpha antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

TOP2A-1361
  • 抗原表位

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    AA 1352-1493

    纯化方法

    Protein G affinity chromatography

    免疫原

    Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

    亚型

    IgG2b kappa
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Topoisomerase II alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

    别名

    TOP2A / Topoisomerase II alpha

    背景

    This mAb recognizes an ~170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

    基因ID

    7153

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases
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