Retinoblastoma Protein (Rb) 抗体 (AA 703-772)
Quick Overview for Retinoblastoma Protein (Rb) 抗体 (AA 703-772) (ABIN4949860)
抗原
See all Retinoblastoma Protein (Rb) (Rb Protein) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 703-772
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纯化方法
- Protein G affinity chromatography
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免疫原
- A human recombinant partial protein was used as the immunogen for this Rb antibody. The epitope is localized within amino acids 703-772.
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亚型
- IgG1 kappa
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anti-Retinoblastoma Protein (Rb Protein) (AA 858-886) antibody
Rb Protein 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) (pSer811) antibodyRb Protein 适用: 人 ELISA, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) (AA 586-615) antibodyRb Protein 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) (Cleavage Site) antibodyRb Protein 适用: 人, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal 172C1094 unconjugated
anti-Retinoblastoma Protein (Rb Protein) (pSer788) antibodyRb Protein 适用: 人 ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) (pSer608) antibodyRb Protein 适用: 人 ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) (pSer780) antibodyRb Protein 适用: 人 ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) (C-Term) antibodyRb Protein 适用: 人, Cow, 犬, Pig, Bat, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) (AA 703-752) antibodyRb Protein 适用: 人, Cow, 犬, 猴, Pig, 兔, Bat, 马, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Retinoblastoma Protein (Rb Protein) antibodyRb Protein 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 7E4B8 unconjugated
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应用备注
- Titering of the Rb antibody may be required for optimal performance.\. WB: 0.5-1 μg/mL,IP: 1-2 μg/500 μg protein lysate,IHC (FFPE): 1-2 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- The Rb antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
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- Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))
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别名
- Retinoblastoma / Rb
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背景
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The retinoblastoma protein (protein name abbreviated pRb, gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases.
Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding-protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki]
mAb 1F8 ecognizes a 105 kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.
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途径
- Mitotic G1-G1/S Phases
抗原
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