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Retinoblastoma Protein (Rb) 抗体 (AA 703-772)

Name: Retinoblastoma Protein (Rb) 抗体 (AA 703-772)
SKU: ABIN4949859
Availability: OutOfStock

This anti-Retinoblastoma Protein (Rb) antibody is a 小鼠单克隆 antibody detecting Retinoblastoma Protein (Rb) in WB, IP 和 IHC (p). Suitable for 人和小鼠.

IHC testing of human colon with Rb antibody (clone 1F8). (Retinoblastoma Protein (Rb) 抗体 (AA 703-772))

IHC

1 image

产品编号 ABIN4949859

发货至: 中国

Datasheet as PDF

Quick Overview for Retinoblastoma Protein (Rb) 抗体 (AA 703-772) (ABIN4949859)

抗原

See all Retinoblastoma Protein (Rb) (Rb Protein) 抗体

Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

适用

人, 小鼠

宿主

小鼠

克隆类型

单克隆

标记

This Retinoblastoma Protein (Rb) antibody is un-conjugated

应用范围

Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

1F8

Discover our top product Rb Protein Primary Antibody

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AA 703-772

纯化方法

Protein G affinity chromatography

免疫原

A human recombinant partial protein was used as the immunogen for this Rb antibody. The epitope is localized within amino acids 703-772.

亚型

IgG1 kappa
anti-Retinoblastoma Protein (Rb Protein) (AA 858-886) antibody
Rb Protein 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) (pSer811) antibody
Rb Protein 适用: 人 ELISA, IHC, IF, DB 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) (AA 586-615) antibody
Rb Protein 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) (Cleavage Site) antibody
Rb Protein 适用: 人, 小鼠 WB, IHC 宿主: 小鼠 Monoclonal 172C1094 unconjugated

anti-Retinoblastoma Protein (Rb Protein) (pSer788) antibody
Rb Protein 适用: 人 ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) (pSer608) antibody
Rb Protein 适用: 人 ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) (pSer780) antibody
Rb Protein 适用: 人 ELISA, IHC, DB 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) (C-Term) antibody
Rb Protein 适用: 人, Cow, 犬, Pig, Bat, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) (AA 703-752) antibody
Rb Protein 适用: 人, Cow, 犬, 猴, Pig, 兔, Bat, 马, 豚鼠 WB 宿主: 兔 Polyclonal unconjugated

anti-Retinoblastoma Protein (Rb Protein) antibody
Rb Protein 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 7E4B8 unconjugated

应用备注

Titering of the Rb antibody may be required for optimal performance.\. WB: 0.5-1 μg/mL,IP: 1-2 μg/500 μg protein lysate,IHC (FFPE): 1-2 μg/mL for 30 min at RT

限制

仅限研究用
浓度

0.2 mg/mL

缓冲液

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

The Rb antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
抗原

Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))

别名

Retinoblastoma / Rb

背景

The retinoblastoma protein (protein name abbreviated pRb, gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases.
Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding-protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki]
mAb 1F8 ecognizes a 105  kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.

途径

Mitotic G1-G1/S Phases