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ENO2/NSE 抗体 (AA 416-433)

This 小鼠 单克隆 anti-ENO2/NSE antibody (Clone ENO2-1375) specifically detects ENO2/NSE in WB, IF, FACS 和 IHC (p). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN4949836
发货至: 中国
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Quick Overview for ENO2/NSE 抗体 (AA 416-433) (ABIN4949836)

抗原

See all ENO2/NSE (ENO2) 抗体
ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

适用

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人, 小鼠, 大鼠

宿主

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小鼠

克隆类型

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单克隆

标记

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This ENO2/NSE antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

ENO2-1375
  • 抗原表位

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    AA 416-433

    纯化方法

    Protein G affinity chromatography

    免疫原

    Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

    亚型

    IgG2b
  • 应用备注

    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

    别名

    NSE / Neuron Specific Enolase

    背景

    Recognizes a protein of about 50  kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

    基因ID

    2026
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