ENO2/NSE 抗体 (AA 416-433)
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Quick Overview for ENO2/NSE 抗体 (AA 416-433) (ABIN4949836)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 416-433
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纯化方法
- Protein G affinity chromatography
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免疫原
- Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.
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亚型
- IgG2b
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应用备注
- The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- NSE / Neuron Specific Enolase
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背景
- Recognizes a protein of about 50 kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
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基因ID
- 2026
抗原
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