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GJB1 抗体 (Cytoplasmic Loop)

This anti-GJB1 antibody is a 小鼠 单克隆 antibody detecting GJB1 in WB, IF, FACS 和 IHC (fro). Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN4949578
发货至: 中国

Quick Overview for GJB1 抗体 (Cytoplasmic Loop) (ABIN4949578)

抗原

See all GJB1 抗体
GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

适用

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人, 大鼠, 小鼠

宿主

  • 55
  • 25
  • 12
小鼠

克隆类型

  • 54
  • 38
单克隆

标记

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This GJB1 antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

克隆位点

M12-13
  • 抗原表位

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    Cytoplasmic Loop

    纯化方法

    Protein G affinity chromatography

    免疫原

    Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

    亚型

    IgG
  • 应用备注

    Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    1 mg/mL in 1X PBS, BSA free, sodium azide free

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原

    GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

    别名

    Connexin 32 / GJB1

    背景

    This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

    途径

    Cell-Cell Junction Organization
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