GJB1 抗体
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Quick Overview for GJB1 抗体 (ABIN4949576)
抗原
See all GJB1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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纯化方法
- Protein G affinity chromatography
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免疫原
- Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.
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亚型
- IgG2a
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应用备注
- Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 1-2 μg/mL for 30 min at RT
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))
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别名
- Connexin 32 / GJB1
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背景
- This Ab recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.
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途径
- Cell-Cell Junction Organization
抗原
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