Phospholipase C gamma 1 抗体 (AA 970-990)
Quick Overview for Phospholipase C gamma 1 抗体 (AA 970-990) (ABIN492624)
抗原
See all Phospholipase C gamma 1 (PLCG1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 970-990
-
特异性
- This monoclonal PLCG antibody (Clone 2D5) specifically recognizes Phospholipase C gamma at 150 kDa in Western blot applications.
-
产品特性
- Synonyms: Phospholipase C gamma, PLC-gamma, Phosphoinositide Phospholipase C, PLC-148, PLC-II,PLC-gamma-1, PLC1, PLCG-1, EC=3.1.4.11, Phospholipase C-gamma-1
-
纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
-
组件
- incl. positive Control
-
免疫原
- Synthetic peptide conjugated to hemocyanin corresponding to amino acids 970-990 ofPLCgamma (Phospholipase C gamma).
-
亚型
- IgG1
-
-
anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibody
PLCG1 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 1091-1290) antibodyPLCG1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (pTyr771) antibodyPLCG1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 1192-1291) antibodyPLCG1 适用: 人, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 3C2D11 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 39-181) antibodyPLCG1 适用: 人, 大鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2F3D11 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (pTyr783) antibodyPLCG1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (pTyr783) antibodyPLCG1 适用: 小鼠, 大鼠 WB, ELISA, IF (p), IHC (p), IF (cc), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (Tyr771) antibodyPLCG1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (AA 456-488) antibodyPLCG1 适用: 人 WB 宿主: 兔 Polyclonal RB53783 unconjugated
anti-phospholipase C, gamma 1 (PLCG1) (pTyr771) antibodyPLCG1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
-
Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control Provided. Cell lysate from untreated SKOV-3 cellsDescription: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
-
限制
- 仅限研究用
-
-
-
溶解方式
- Restore with 1 mL H2O (15 min, RT).
-
缓冲液
- 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
-
储存液
- Sodium azide
-
注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- -20 °C
-
储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
-
有效期
- 12 months
-
-
- Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))
-
别名
- PLCG1
-
背景
- Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLCgamma at Tyr 771, 783 and 1245. Phosphorylation of Tyr 783 leads to enzymatic activation of PLCgamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).Synonyms: EC=3.1.4.11, PLC-148, PLC-II, PLC-gamma, PLC-gamma-1, PLC1, PLCG-1, Phosphoinositide Phospholipase C, Phospholipase C gamma, Phospholipase C-gamma-1
-
基因ID
- 5335
-
UniProt
- P19174
-
途径
- RTK signaling, WNT signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling
抗原
-
