PAK4 抗体
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北京 101111
Quick Overview for PAK4 抗体 (ABIN492621)
抗原
See all PAK4 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes p21 activated kinase 4 (PAK4).
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产品特性
- Synonyms: PAK 4, PAK-4, p21-activated kinase 4, KIAA1142, Serine/threonine-protein kinase PAK 4
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纯化方法
- Subsequent Ultrafiltration and Size Exclusion Chromatography.
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组件
- incl. positive Control
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免疫原
- Peptide conjugated to hemocyaninEpitope: Peptide derived from the kinase activation loop.
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亚型
- IgG1
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anti-P21-Activated Kinase 4 (PAK4) (AA 299-542) antibody
PAK4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (AA 1-426) antibodyPAK4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (AA 187-216) antibodyPAK4 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (AA 120-280) antibodyPAK4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (pSer474), (pSer560), (pSer602) antibodyPAK4 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 2D1 unconjugated Recombinant Antibody
anti-P21-Activated Kinase 4 (PAK4) (AA 187-216) antibodyPAK4 适用: 人, 小鼠 WB, IHC, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (AA 23-72) antibodyPAK4 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 马, 斑马鱼, Bat, Hamster, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (AA 156-187) antibodyPAK4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (AA 187-216) antibodyPAK4 适用: 人, 小鼠 WB, FACS 宿主: 兔 Polyclonal unconjugated
anti-P21-Activated Kinase 4 (PAK4) (pSer474) antibodyPAK4 适用: 人, 小鼠, 大鼠, Cow WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW480 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- Positive Control Provided. Cell lysate from untreated SW480 cellsDescription: Cell lysate from untreated SW480 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SW480 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
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限制
- 仅限研究用
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溶解方式
- Restore with 1 mL H2O (15 min, RT).
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缓冲液
- 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
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有效期
- 12 months
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- PAK4 (P21-Activated Kinase 4 (PAK4))
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别名
- PAK4
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背景
- P21 activated kinases (PAK) are regulated by the small GTP - binding proteins Rac and cdc42, which stimulate autophosphorylation and phosphorylation of downstream signaling proteins. PAK4 is activated upon autophosphorylation of Ser 474 in the kinase activation loop. PAK4 is frequently overexpressed in human tumor cell lines and might play an important role in oncogenesis.Synonyms: KIAA1142, PAK 4, PAK-4, Serine/threonine-protein kinase PAK 4, p21-activated kinase 4
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基因ID
- 10298
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UniProt
- O96013
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途径
- RTK signaling
抗原
-
