(1 reference)-
- 抗原 See all PSMA (FOLH1) 抗体
- PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This PSMA antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody reacts with PSMA (100 kDa) on Flow Cytometry.
- 交叉反应 （详细）
- Species reactivity (tested):Human.
- 产品特性
- Synonyms: Glutamate carboxypeptidase 2, Folate hydrolase 1, Prostate-specific membrane antigen,FOLH, NAALAD1, PSM, GCP2, NAALAdase, Glutamate carboxypeptidase II, Membraneglutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I,Pteroylpoly-gamma-glutamate carboxypeptidase, Folylpoly-gamma-glutamatecarboxypeptidase
- 纯化方法
- Protein-A Agarose Chromatography of hybridoma supernatant.
- 免疫原
- LNCaP cell homogenate.
- 克隆位点
- 107-1A4
- 亚型
- IgG1
- Top Product
- Discover our top product FOLH1 Primary Antibody
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anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 44-750) antibody
FOLH1 适用: 人, 小鼠, 大鼠, Pig WB, IHC (p), ICC 宿主: 小鼠 Monoclonal GCP-04 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) antibodyFOLH1 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 274-587) antibodyFOLH1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 44-177) antibodyFOLH1 适用: 人 WB, IHC, ELISA, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 2D10E10 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 275-588) antibodyFOLH1 适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 232-433) antibodyFOLH1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal FOLH1-2354 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 44-750) antibodyFOLH1 适用: 人 FACS, ICC, IP, CyTOF 宿主: 小鼠 Monoclonal GCP-05 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 161-190) antibodyFOLH1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) (AA 1-719) antibodyFOLH1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B5 unconjugated
anti-Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1) antibodyFOLH1 适用: 人, 小鼠, 大鼠, 犬, 猴 WB, IF 宿主: 小鼠 Monoclonal 2E1 unconjugated
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- 应用备注
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Flow Cytometry: 10 μg/mL (final concentration). Positive Control: LNCaP cell. Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- Flow Cytometric analysis for for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all steps described below. 1) Detach the cells from culture dish by using cell dissociation buffer2) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN]. 3) Resuspend the cells with washing buffer (5x10e6 cells/mL). 4) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 5) Add 20 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 6) Add 30 µL of the primary antibody at the concentration of as suggest in theAPPLICATIONS diluted in the washing buffer. Mix well and incubate for 30 minutes at roomtemperature. 7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 8) Add 30 µL of 1: 100 FITC conjugated anti-mouse IgG diluted with the washing buffer. Mixwell and incubate for 15 minutes at room temperature. 9) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature. Remove supernatant by careful aspiration. 10) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: LNCaP
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- PBS containing 1 % BSA and 0.09 % Sodium Azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch. - 有效期
- 12 months
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: "Heterogeneous PSMA expression on circulating tumor cells: a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer." in: Oncotarget, Vol. 7, Issue 23, pp. 34930-41, (2018) (PubMed).
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: "Heterogeneous PSMA expression on circulating tumor cells: a potential basis for stratification and monitoring of PSMA-directed therapies in prostate cancer." in: Oncotarget, Vol. 7, Issue 23, pp. 34930-41, (2018) (PubMed).
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- 抗原
- PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))
- 别名
- PSMA / FOLH1 (FOLH1 产品)
- 别名
- FGCP antibody, FOLH antibody, GCP2 antibody, GCPII antibody, NAALAD1 antibody, NAALAdase antibody, PSM antibody, PSMA antibody, mGCP antibody, mopsm antibody, Naalad antibody, putative N-acetylated-alpha-linked acidic dipeptidase antibody, folate hydrolase 1B antibody, folate hydrolase 1 antibody, folate hydrolase (prostate-specific membrane antigen) 1 antibody, LOC451185 antibody, FOLH1B antibody, FOLH1 antibody, Folh1 antibody
- 背景
- PSMA is a type II transmembrane protein expressed at high levels in prostate cancer and in tumor-associated neovasculature. PSMA is also known as a glutamate carboxypeptidase II (EC 3.4.17.21) that hydrolyzes the neuropeptide, N-acetyl-L-aspartyl-L-glutamate, releasing glutamate, the dominant excitatory neurotransmitter/neuromodulator of the mammalian central nervous system. The ability of PSMA to liberate g-glutamate may allow the removal of glutamate residues from dietary folates, which are unable to be transported into cells in their poly-g-glutamate form. Overexpression of PSMA in prostate cancer cells may represent an advantageous adaptation that allows the uptake of folates required for rapid division. The exopeptidase activity exhibited by PSMA and its high-level overexpression in prostate cancer cells make it a potential target for selective anticancer therapy and prodrug activation.Synonyms: FOLH, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, GCP2, Glutamate carboxypeptidase 2, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, NAALAD1, NAALAdase, PSM, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase
- 基因ID
- 2346
- UniProt
- Q04609
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