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ERBB3 抗体 (C-Term) (Biotin)

This anti-ERBB3 antibody is a 小鼠单克隆 antibody detecting ERBB3 in WB. Suitable for 人.

产品编号 ABIN492350

发货至: 中国

Quick Overview for ERBB3 抗体 (C-Term) (Biotin) (ABIN492350)

抗原

See all ERBB3 抗体

ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

适用

164
104
89
17
6
4
3
3
3
2
2
1
1
1

人

宿主

小鼠

克隆类型

单克隆

标记

113
14
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
1
1

This ERBB3 antibody is conjugated to Biotin

应用范围

104
72
53
53
53
31
29
21
20
15
11
3
3
3
2
1

Western Blotting (WB)

克隆位点

11A4

Discover our top product ERBB3 Primary Antibody

抗原表位
27

26

15

15

14

9

7

7

6

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term

特异性

This antibody specifically recognizes the C-terminus of erbB3 at 185 kDa.

产品特性

Synonyms: ERBB-3, c-erbB-3, HER-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cellsurface receptor HER3, Proto-oncogene-like protein c-ErbB-3

纯化方法

Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

组件

incl. positive Control

免疫原

Synthetic petide conjugated to KLH. Epitope: C-terminus

亚型

IgG1
anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 707-964) antibody
ERBB3 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 24-55), (N-Term) antibody
ERBB3 适用: 人, 小鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal RB01467 unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (pTyr1289) antibody
ERBB3 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 1-130) antibody
ERBB3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (C-Term) antibody
ERBB3 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 24-55) antibody
ERBB3 适用: 人, 小鼠 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (pTyr1328) antibody
ERBB3 适用: 人, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 661-760) antibody
ERBB3 适用: 人, 小鼠, 大鼠 ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 21-332) antibody
ERBB3 适用: 人 IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3F12 unconjugated

anti-Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3) (AA 1-131) antibody
ERBB3 适用: 人 WB, IHC, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2E9 unconjugated

应用备注

Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SW620 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

实验流程

Positive Control Provided. Cell lysate from untreated SW620Description: Cell lysate from untreated SW620 cells, colorectal adenocarcinoma (Human)Format: Lyophilized cell lysate from starved cells SW 620 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

限制

仅限研究用
浓度

0.5 mg/mL

缓冲液

PBS, 0.09 % Sodium Azide/PEG and Sucrose.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C

储存方法

Aliquote and freeze in liquid nitrogenAntibody can be stored frozen at -80 °C up to 1 year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.

有效期

12 months
抗原

ERBB3 (Receptor Tyrosine-Protein Kinase ErbB-3 (ERBB3))

别名

ERBB3 / HER3

背景

ErbB3 is a member of the EGFR/erbB receptor tyrosine kinase family. ErbB3 lacks intrinsic kinase activity due to an amino acid substitution within the ATP binding site. Upon heterodimerization with the other erbB family members, erbB3 becomes phosphorylated at multiple tyrosine residues that represent docking sites for downstream signaling proteins, e.g. PI3K, grb7 and shc. The erbB3 cytoplasmic domain contains 6 docking sites for PI3K. Thus, erbB3 is a potent activator of the PI3K/PKB signaling pathway.Synonyms: ERBB-3, HER-3, Proto-oncogene-like protein c-ErbB-3, Receptor tyrosine-protein kinase erbB-3, Tyrosine kinase-type cell surface receptor HER3, c-erbB-3

基因ID

2065

UniProt

P21860

途径

RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

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