Acetylated Lysine 抗体 (acetylated, Lys25)
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Quick Overview for Acetylated Lysine 抗体 (acetylated, Lys25) (ABIN488677)
抗原
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- acetylated, Lys25
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原理
- Anti-Acetylated lysine (25 μl, clone 7F8)
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特异性
- The antibody detects proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
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交叉反应 （详细）
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Bovine, avian
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
预测反应
- Higher plants
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纯化方法
- Protein G purified
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免疫原
- acetylated KLH
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亚型
- IgG1
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anti-Acetylated Lysine antibody
适用: Various Species WB, ELISA, IHC, IP, FACS, FM 宿主: 兔 Polyclonal unconjugated
anti-Acetylated Lysine antibody适用: Cow, 小鸡 WB, ELISA, IHC (p), IHC (fro) 宿主: 小鼠 Monoclonal 7F8 unconjugated
anti-Acetylated Lysine antibodyWB 宿主: 小鼠 Monoclonal 15G10 unconjugated
anti-Acetylated Lysine (acetylated) antibodyWB, IP, IF, EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
- 1 : 100 (IHC), 1 : 1000 (WB)
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说明
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1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Total IgG fraction. Protein G purified.
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注意事项
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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储存条件
- -20 °C
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储存方法
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Acetylated Lysine
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物质类
- Chemical
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背景
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Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Additional information: Protein G purified IgG2B in PBS, pH 7,4 with 0,09 % sodium azide and 50 % glycerol at concentration 1 mg/mLantibody detects Proteins containing acetylated lysine residues in ELISA and WBs, Does not detect non-acetylated lysine residues
抗原
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