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Acetylated Lysine 抗体

WB, ELISA, IP, IHC, FACS, FM 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN5596809
发货至: 中国
  • 抗原 See all Acetylated Lysine products
    Acetylated Lysine
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This Acetylated Lysine antibody is un-conjugated
    应用范围
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    Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    交叉反应 (详细)
    The antibody reacts specifically with acetylated lysine residues.
    纯化方法
    Anti-Lysine Acetylated Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using acetylated lysine peptide coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.
    免疫原

    Immunogen: Anti-Lysine Acetylated Antibody was prepared from whole rabbit serum produced by repeated immunizations with acetylated lysine conjugated KLH.

    Immunogen Type: Other

    亚型
    IgG
  • 应用备注

    Flow Cytometry Dilution: User Optimized

    Immunohistochemistry Dilution: User Optimized

    Application Note: Anti-Lysine Acetylated Antibody is suitable for use in ELISA, western blotting, immunofluorescence microscopy, and immunoprecipitation assays. Although not tested, this antibody is likely functional in RIA, flow cytometry, and immunohistochemistry.

    Western Blot Dilution: 1:500-1:1,000

    Immunoprecipitation Dilution: 10 μg/mg protein sample

    ELISA Dilution: 1:1,000-1:2,500

    IF Microscopy Dilution: User Optimized

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1.0 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    12 months
  • Zhou, Huang, Zhang, Zhao, Kim, Tu, Zhang, Nowsheen, Zhu, Deng, Chen, Qin, Luo, Liu, Lou, Mutter, Yuan: "The bromodomain containing protein BRD-9 orchestrates RAD51-RAD54 complex formation and regulates homologous recombination-mediated repair." in: Nature communications, Vol. 11, Issue 1, pp. 2639, (2020) (PubMed).

    Lin, Yuan, Pei, Liu, Ann, Lou: "KAP1 Deacetylation by SIRT1 Promotes Non-Homologous End-Joining Repair." in: PLoS ONE, Vol. 10, Issue 4, pp. e0123935, (2016) (PubMed).

    Pérez-Luna, Aguasca, Perearnau, Serratosa, Martínez-Balbas, Jesús Pujol, Bachs: "PCAF regulates the stability of the transcriptional regulator and cyclin-dependent kinase inhibitor p27 Kip1." in: Nucleic acids research, Vol. 40, Issue 14, pp. 6520-33, (2012) (PubMed).

  • 抗原
    Acetylated Lysine
    别名
    Lysine Acetylated (Acetylated Lysine 产品)
    别名
    C-K-RAS antibody, CFC2 antibody, K-RAS2A antibody, K-RAS2B antibody, K-RAS4A antibody, K-RAS4B antibody, KI-RAS antibody, KRAS1 antibody, KRAS2 antibody, NS antibody, NS3 antibody, RASK2 antibody, KRAS proto-oncogene, GTPase antibody, KRAS antibody
    物质类
    Chemical
    背景

    Synonyms: acetyl Lysine antibody, Acetylated lysine antibody, Lysine antibody

    Background: Acetylation refers to the process of introducing an acetyl group into a compound. Proteins are typically acetylated on lysine residues and this reaction relies on acetyl-coenzyme A as the acetyl group donor. Acetylated Lysine antibody is useful in Cancer, Cell Biology and Chromatin & Nuclear Signaling research.

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