NAMPT 抗体 (AA 62-337)
Quick Overview for NAMPT 抗体 (AA 62-337) (ABIN4886677)
抗原
See all NAMPT 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 62-337
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原理
- Anti-Visfatin/NAMPT Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Visfatin/NAMPT Antibody Picoband® (ABIN4886677). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E. coli-derived human Visfatin recombinant protein (Position: L62-N337). Human Visfatin shares 97.1% and 96.7% amino acid (aa) sequence identity with mouse and rat Visfatin, respectively.
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亚型
- IgG
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anti-Nicotinamide phosphoribosyltransferase (NAMPT) antibody
NAMPT 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IP, ICC, FACS 宿主: 小鼠 Monoclonal OMNI379 unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) antibodyNAMPT 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) antibody (Biotin)NAMPT 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal OMNI379 Biotin
anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 301-400) antibodyNAMPT 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-280) antibodyNAMPT 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 400-426) antibodyNAMPT 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-491) antibodyNAMPT 适用: 人 WB 宿主: 小鼠 Monoclonal D1 unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) (AA 1-280) antibodyNAMPT 适用: 人 ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) antibodyNAMPT 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nicotinamide phosphoribosyltransferase (NAMPT) (C-Term) antibodyNAMPT 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, ICC, IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: PBEF1 pre-B-cell colony enhancing factor 1". 2. Samal B, Sun Y, Stearns G, Xie C, Suggs S, McNiece I (February 1994). "Cloning and characterization of the cDNA encoding a novel human pre-B-cell colony-enhancing factor". Mol. Cell. Biol. 14 (2): 1431-7. 3. Wang T, Zhang X, Bheda P, Revollo JR, Imai S, Wolberger C (July 2006). "Structure of Nampt/PBEF/visfatin, a mammalian NAD+ biosynthetic enzyme". Nat. Struct. Mol. Biol. 13 (7): 661-2. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))
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别名
- NAMPT
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背景
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Synonyms: Nicotinamide phosphoribosyltransferase,NAmPRTase,Nampt,2.4.2.12,Pre-B-cell colony-enhancing factor 1,Pre-B cell-enhancing factor,Visfatin,NAMPT,PBEF, PBEF1,
Tissue Specificity: Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
Background: Nicotinamide phosphoribosyltransferase (NAmPRTase or Nampt), also known as pre-B-cell colony-enhancing factor 1 (PBEF1) or visfatin, is an enzyme that in humans is encoded by the PBEF1 gene. This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.
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分子量
- 54 kDa
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基因ID
- 10135
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UniProt
- P43490
抗原
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