alpha Actinin 4 抗体 (AA 561-661)
Quick Overview for alpha Actinin 4 抗体 (AA 561-661) (ABIN4886421)
抗原
See all alpha Actinin 4 (ACTN4) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 561-661
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原理
- Anti-Alpha Actinin 4/ACTN4 Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-Alpha Actinin 4/ACTN4 Antibody Picoband® (ABIN4886421). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human Alpha Actinin 4 recombinant protein (Position: E561-V661). Human Alpha Actinin 4 shares 99% and 98% amino acid (aa) sequence identity with mouse and rat Alpha Actinin 4, respectively.
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亚型
- IgG
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anti-Actinin, alpha 4 (ACTN4) (AA 592-701) antibody
ACTN4 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4D10 unconjugated
anti-Actinin, alpha 4 (ACTN4) (AA 1-269) antibodyACTN4 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actinin, alpha 4 (ACTN4) (C-Term) antibodyACTN4 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Actinin, alpha 4 (ACTN4) antibodyACTN4 适用: 人 WB, IP, IF 宿主: 兔 Monoclonal unconjugated
anti-Actinin, alpha 4 (ACTN4) (AA 160-440) antibodyACTN4 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actinin, alpha 4 (ACTN4) (N-Term) antibodyACTN4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 斑马鱼, 马, 兔 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actinin, alpha 4 (ACTN4) antibodyACTN4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actinin, alpha 4 (ACTN4) (AA 1-911) antibodyACTN4 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated
anti-Actinin, alpha 4 (ACTN4) (AA 785-813), (C-Term) antibodyACTN4 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15705 unconjugated
anti-Actinin, alpha 4 (ACTN4) (C-Term) antibodyACTN4 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human, Mouse, Rat
1. "Entrez Gene: ACTN4 actinin, alpha 4". 2. Kaplan, J. M., Kim, S. H., North, K. N., Rennke, H., Correia, L. A., Tong, H.-Q., Mathis, B. J., Rodriguez-Perez, J.-C., Allen, P. G., Beggs, A. H., Pollak, M. R. Mutations in ACTN4, encoding alpha-actinin-4, cause familial focal segmental glomerulosclerosis.Nature Genet. 24: 251-256, 2000. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- alpha Actinin 4 (ACTN4) (Actinin, alpha 4 (ACTN4))
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别名
- ACTN4
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背景
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Synonyms: Alpha-actinin-4 ,Non-muscle alpha-actinin 4 ,ACTN4 ,
Tissue Specificity: Widely expressed. .
Background: Alpha-actinin-4 is a protein that in humans is encoded by the ACTN4 gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This ACTN4 gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.
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分子量
- 105 kDa
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基因ID
- 81
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UniProt
- O43707
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途径
- Proton Transport
抗原
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