Bromelain 抗体 (Biotin)
适用: Pineapple
ELISA, WB, IF, IHC
宿主: 兔
Polyclonal
Biotin
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- 抗原
- Bromelain
- 适用
- Pineapple
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宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- Biotin
- 应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 预测反应
- Ananas comosus
- 产品特性
- Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radialimmunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition.
- 纯化方法
- affinity purified
- 免疫原
- native bromelain isolated and purified from Ananas comosus
- 亚型
- IgG
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-
- 应用备注
- Recommended Dilution: 1 : 5000 - 1 : 50 000 for techniques listed above.
- 说明
-
Biotin/IgG protein molar ration is approximately 6.2. No foreign proteins are added.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- For reconstitution please add 1 mL of sterile destilled water.
- 缓冲液
- phosphate buffer saline
- 注意事项
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- 储存条件
- -20 °C
-
- 抗原
- Bromelain
- 背景
- Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
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