Bromelain 抗体
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- 抗原
- Bromelain
- 适用
- Pineapple
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- 非结合性
- 应用范围
- ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
- 预测反应
- Ananas comosus
- 产品特性
- Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.
- 纯化方法
- affinity purified
- 免疫原
- native bromelain isolated and purified from Ananas comosus
- 亚型
- IgG
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- 应用备注
- Recommended Dilution: 1 : 1000-1 : 100 000 (ELISA), (IF), (IHC), (WB)
- 说明
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The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- For reconstitution please add 1 mL of sterile destilled water.
- 缓冲液
- phosphate buffer saline
- 注意事项
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
- 储存条件
- -20 °C
- 储存方法
- Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- 抗原
- Bromelain
- 背景
- Bromelain (EC=3.4.22.32) is a cysteine protease isolated from pineapple stem.
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