Rhodopsin 抗体 (N-Term)
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Quick Overview for Rhodopsin 抗体 (N-Term) (ABIN487510)
抗原
See all Rhodopsin (RHO) 抗体宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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特异性
- N-terminus of Rhodopsin.
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交叉反应 （详细）
- Species reactivity (tested):All Mammalian species (Human, Mouse, Rat, Bovine, Pig, etc), Most Fish, Birds, Amphibians (Frogs, Xenopus), Anchovies. Not Drosophila or other invertebrates.
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产品特性
- Synonyms: RHO, Opsin-2, OPN2
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纯化方法
- Protein G Chromatography.
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免疫原
- Bovine Rhodopsin.
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亚型
- IgG1
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anti-Rhodopsin (RHO) antibody
RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Rhodopsin (RHO) antibody (PerCP)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 PerCP
anti-Rhodopsin (RHO) antibody (APC)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 APC
anti-Rhodopsin (RHO) antibody (FITC)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 FITC
anti-Rhodopsin (RHO) antibody (Biotin)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Biotin
anti-Rhodopsin (RHO) antibody (Atto 488)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 488
anti-Rhodopsin (RHO) antibody (Atto 594)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 594
anti-Rhodopsin (RHO) antibody (Atto 390)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 Atto 390
anti-Rhodopsin (RHO) antibody (HRP)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 HRP
anti-Rhodopsin (RHO) antibody (PE)RHO 适用: Cow WB, IHC, ELISA, IP, ICC, IF 宿主: 小鼠 Monoclonal 4D2 PE
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应用备注
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ELISA. Western blot: 1/1000 (1)1 μg/mL was sufficient for detection of Rhodopsin in 10 μg of Rat eye lysate by colorimetricimmunoblot analysis using Goat anti-mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1-2 μg per 100-500 μg of proteinImmunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS pH 7.4 containing 50 % Glycerol and 0.09 % Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- Rhodopsin (RHO)
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别名
- Rhodopsin
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物质类
- Chemical
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背景
- Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photoreactive chromophore, in a central pocket (2, 3). In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2). In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4). Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5).Synonyms: OPN2, Opsin-2, RHO
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基因ID
- 6010
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UniProt
- P08100
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途径
- WNT signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction
抗原
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