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FZD1 抗体 (Extracellular Domain) (Biotin)

This anti-FZD1 antibody is a 大鼠 单克隆 antibody detecting FZD1 in WB, ICC 和 FACS. Suitable for 人 和 小鼠.
产品编号 ABIN478743
发货至: 中国

Quick Overview for FZD1 抗体 (Extracellular Domain) (Biotin) (ABIN478743)

抗原

See all FZD1 (Fzd1) 抗体
FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

适用

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人, 小鼠

宿主

  • 37
  • 4
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  • 2
大鼠

克隆类型

  • 40
  • 6
单克隆

标记

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This FZD1 antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • 抗原表位

    • 5
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    Extracellular Domain

    特异性

    Recognizes mouse Frizzled-1 extracellular domain. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry. Species cross-reactivity: Human.

    纯化方法

    Protein G purified

    免疫原

    NS0-derived rmFrizzled-1 extracellular domain.

    亚型

    IgG2a
  • 应用备注

    Approved: Flo, ICC, WB

    Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 10 μL of this solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 mL of a 25 mg/mL stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome. Immunocytochemistry: Used with appropriate secondary reagents at 5-10 μg/mL in 4 % . paraformaldehyde-fixed cells. Western Blot: Use at 1-2 μg/mL with appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Mouse

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.2, BSA.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: 4°C
    Long term: Add glycerol (40-50%) -20°C.
  • 抗原

    FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))

    别名

    FZD1 / Frizzled 1

    背景

    Name/Gene ID: FZD1
    Subfamily: Frizzled
    Family: GPCR

    Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1

    基因ID

    8321

    UniProt

    Q9UP38

    途径

    WNT signaling, Asymmetric Protein Localization
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