FZD1 抗体 (Extracellular Domain) (Biotin)
Quick Overview for FZD1 抗体 (Extracellular Domain) (Biotin) (ABIN478743)
抗原
See all FZD1 (Fzd1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Extracellular Domain
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特异性
- Recognizes mouse Frizzled-1 extracellular domain. Shows no cross-reactivity with rmFrizzled-2, -3, -4, -6, -7 and -8. Detected Frizzled-1 on differentiated embryoid bodies derived from mouse embryonic cell line D3 by Flow Cytometry. Species cross-reactivity: Human.
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纯化方法
- Protein G purified
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免疫原
- NS0-derived rmFrizzled-1 extracellular domain.
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亚型
- IgG2a
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应用备注
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Approved: Flo, ICC, WB
Usage: Suitable for use in Flow Cytometry, Immunocytochemistry and Western Blot. Flow Cytometry: Dilute this antibody to 0.1 mg/mL and add 10 μL of this solution to 1-2.5x10^5 cells in a total reaction volume not exceeding 200 μL. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 mL of a 25 mg/mL stock solution of a secondary developing reagent such as streptavidin conjugated to a fluorochrome. Immunocytochemistry: Used with appropriate secondary reagents at 5-10 μg/mL in 4 % . paraformaldehyde-fixed cells. Western Blot: Use at 1-2 μg/mL with appropriate secondary reagents to detect mouse Frizzled-1. The detection limit for rmFrizzled-1 is ~5 ng/lane under non-reducing and reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Mouse
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, BSA.
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储存条件
- 4 °C,-20 °C
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储存方法
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- FZD1 (Fzd1) (Frizzled Family Receptor 1 (Fzd1))
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别名
- FZD1 / Frizzled 1
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背景
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Name/Gene ID: FZD1
Subfamily: Frizzled
Family: GPCR
Synonyms: FZD1, Chfz-1, Fz1, Frizzled 1, Fz receptor 1, HFz1, Frizzled homolog 1, Fz-1, FzE1, Rfz1, Xfrizzled-1, Wnt receptor, Dfrizzled-1, Frizzled family receptor 1, Frizzled-1 -
基因ID
- 8321
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UniProt
- Q9UP38
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途径
- WNT signaling, Asymmetric Protein Localization
抗原
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