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BDNF 抗体 (Biotin)

This anti-BDNF antibody is a 兔 多克隆 antibody detecting BDNF in WB, ELISA 和 Neut. Suitable for 人, 大鼠 和 小鼠.
产品编号 ABIN465696
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for BDNF 抗体 (Biotin) (ABIN465696)

抗原

See all BDNF 抗体
BDNF (Brain-Derived Neurotrophic Factor (BDNF))

适用

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人, 大鼠, 小鼠

宿主

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克隆类型

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多克隆

标记

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This BDNF antibody is conjugated to Biotin

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 特异性

    Human BDNF

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human BDNF.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

    Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hBDNF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human BDNF (LS-C104728) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Sterile PBS containing 0.1 % BSA

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    BDNF (Brain-Derived Neurotrophic Factor (BDNF))

    别名

    BDNF

    背景

    Name/Gene ID: BDNF
    Family: NGF Beta

    Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin

    基因ID

    627

    UniProt

    P23560

    途径

    RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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