BDNF 抗体
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北京 101111
Quick Overview for BDNF 抗体 (ABIN465693)
抗原
See all BDNF 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human BDNF
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纯化方法
- Immunoaffinity purified
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免疫原
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E.coli derived recombinant Human BDNF.
Type of Immunogen: Cell extract
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anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibody
BDNF 适用: Pig WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 19-248) antibodyBDNF 适用: 人 WB, ICC, ELISA 宿主: 小鼠 Monoclonal 3D9C5 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 151-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, ICC, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 20-252) antibodyBDNF 适用: Pig WB, IHC, ICC, IP 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (Internal Region) antibodyVerified BDNF 适用: 人 ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) antibodyBDNF 适用: 人 WB, IP, ELISA, IF 宿主: 小鼠 Monoclonal 1B10 unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 206-236) antibodyBDNF 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) antibodyBDNF 适用: 人, 大鼠, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Brain-Derived Neurotrophic Factor (BDNF) (AA 206-236), (C-Term) antibodyBDNF 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB14839 unconjugated
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应用备注
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Approved: ELISA (0.5 - 2 μg/mL), Neut (0.54 - 0.81 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2 μg/mL overnight at 4 °C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hBDNF (2 μg/mL), a concentration of 0.54-0.81 μg/mL of this antibody is required. ELISA: To detect hBDNF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BDNF (LS-C104729) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBDNF. Western Blot: To detect hBDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBDNF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with sterile water.
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浓度
- Lot specific
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缓冲液
- Lyophilized, PBS, pH 7.2. Reconstitute with sterile water.
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注意事项
- avoid freeze thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
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别名
- BDNF
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背景
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Name/Gene ID: BDNF
Family: NGF Beta
Synonyms: BDNF, ANON2, Abrineurin, BULN2, Neurotrophin -
基因ID
- 627
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UniProt
- P23560
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途径
- RTK signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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