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G-CSF 抗体

This 兔 多克隆 antibody specifically detects G-CSF in WB, ELISA 和 Neut. It exhibits reactivity toward 人.
产品编号 ABIN465577
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for G-CSF 抗体 (ABIN465577)

抗原

See all G-CSF (CSF3) 抗体
G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

适用

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克隆类型

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多克隆

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This G-CSF antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Neutralization (Neut)
  • 特异性

    Human G-CSF

    纯化方法

    Immunoaffinity purified

    免疫原

    E.coli derived recombinant Human G-CSF.

    Type of Immunogen: Cell extract
  • 应用备注

    Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB

    Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human angiosarcoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hG-CSF (0.5 ng/mL), a concentration of 0.04-0.06 μg/mL of this antibody is required. ELISA: To detect hG-CSF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human G-CSF (LS-C104674) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hG-CSF. Western Blot: To detect hG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hG-CSF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Lyophilized

    溶解方式

    Reconstitute with sterile water

    浓度

    Lot specific

    缓冲液

    Lyophilized from PBS,  pH 7.2

    注意事项

    Avoid freeze/thaw cycles.

    储存条件

    4 °C,-20 °C

    储存方法

    Store Lyophilized at room temperature up to 1 month
    Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
  • 抗原

    G-CSF (CSF3) (Colony Stimulating Factor 3 (Granulocyte) (CSF3))

    别名

    CSF3 / G-CSF / GCSF

    背景

    Name/Gene ID: CSF3

    Synonyms: CSF3, C17orf33, Filgrastim, G-CSF, GCSF, Pluripoietin, CSF3OS, Lenograstim

    基因ID

    1440

    UniProt

    P09919

    途径

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization
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