VEGFA 抗体
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北京 101111
Quick Overview for VEGFA 抗体 (ABIN465162)
抗原
See all VEGFA 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Human VEGF
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纯化方法
- Protein A purified
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免疫原
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E.coli derived recombinant Human VEGF165.
Type of Immunogen: Cell extract -
亚型
- IgG1
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应用备注
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Approved: ELISA (2 - 4 μg/mL), WB (0.25 - 0.5 μg/mL)
Usage: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human VEGF (10 ng/mL), a concentration of &le. 0.2 μg/mL of this antibody is required. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 2-4 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human VEGF165 when used with biotinylated antigen affinity purified anti-human VEGF165 (LS-C104615) as the detection antibody at a concentration of approximately 0.25-1 μg/mL. Western Blot: To detect hVEGF165 by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hVEGF165 is 0.25-0.50 ng/lane, under non-reducing conditions. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
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浓度
- Lot specific
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缓冲液
- Lyophilized from PBS
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注意事项
- Avoid freeze/thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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别名
- VEGFA / VEGF
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背景
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Name/Gene ID: VEGFA
Family: PDGF
Synonyms: VEGFA, VPF, Vascular permeability factor, VEGF, VEGF-A, MVCD1 -
基因ID
- 7422
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UniProt
- P15692
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途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
抗原
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