CAMK2B 抗体
Quick Overview for CAMK2B 抗体 (ABIN462429)
抗原
See all CAMK2B 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Detects proteins of 50-60 kD, corresponding to apparent molecular mass of CaMKII isoforms on SDS-PAGE immunoblots. Recombinant rat calmodulin-dependent protein kinase II alpha subunits expressed in sf9 insect cells are also detected. As the sequences of the rat alpha, delta, and gamma isoforms are conserved over this amino terminus region, this antibody is expected to recognize the delta, gamma and alpha isoforms. This antibody is not expected to cross-react with the beta isoform. Proteins of unknown identity, ~40, 45 and 90 kD, may be detected on immunoblot analysis with some lysates.
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预测反应
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%) Orangutan (93%).
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纯化方法
- Immunoaffinity purified
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免疫原
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A 14 residue synthetic peptide CTRFTDEYQLFEEL based on the rat multifunctional calmodulin-dependent kinase II delta isoform (residues 7-20) and the peptide coupled to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Horse, Rabbit, Pig, Opossum, Chicken, Xenopus, Pufferfish, Zebrafish (100%), Orangutan (93%).
Type of Immunogen: Synthetic peptide - KLH conjugated
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anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (AA 1-100) antibody
CAMK2B 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (pThr286) antibodyCAMK2B 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) antibodyCAMK2B 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (AA 101-200) antibodyCAMK2B 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (AA 1-504) antibodyCAMK2B 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal S11 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (AA 1-503) antibodyCAMK2B 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal S11 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (AA 1-504) antibodyCAMK2B 适用: 人 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal S11 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (pThr287) antibodyCAMK2B 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (AA 405-502) antibodyCAMK2B 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 6D6 unconjugated
anti-Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B) (pThr286), (Thr287) antibodyCAMK2B 适用: 大鼠 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 22B1 unconjugated
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应用备注
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Approved: IHC, IHC-P (10 μg/mL), WB (1:1000)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
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Target Species of Antibody: Rat
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- BBS, pH 8.2 (25 mM sodium borate, 100 mM boric acid, 75 mM sodium chloride, 5 mM EDTA)
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储存条件
- -20 °C
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储存方法
- Store at -20°C.
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- CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))
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别名
- CAMK2 / CAMKII
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背景
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Name/Gene ID: CAMK2
Synonyms: CAMK2 -
途径
- WNT signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
抗原
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