HSPD1 抗体
Quick Overview for HSPD1 抗体 (ABIN462364)
抗原
See all HSPD1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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品牌
- IHC-plus™
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特异性
- Detects an ~60 kD protein, corresponding to the apparent molecular mass of Hsp60 on SDS-PAGE immunoblots, in samples from human, mouse, rat, bovine, chicken, dog, guinea pig, hamster, monkey, pig, rabbit, plant (spinach), Borrelia, E. coli, helicobacter pylori, M. bovis (Hsp65), Salmonella typhimurium, Streptococcus pyogenes, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Yersinia entercolitica, and yeast origins. Deletion mutant analysis indicates that the epitope recognized by LK-2 is located between residues 383-419 of the human Hsp60 sequence (which corresponds to residues 356-393 of the mycobacterial Hsp60 sequence).
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纯化方法
- Protein G purified
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免疫原
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Human HSP60 produced through recombinant DNA methods in E. coli.
Type of Immunogen: Recombinant protein -
亚型
- IgG1
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anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 501-573) antibody
HSPD1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 27-240) antibodyHSPD1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人 WB, IHC, FACS, ELISA, IP 宿主: 小鼠 Monoclonal LK1 unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 3G8 unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 172-343) antibodyHSPD1 适用: 人 WB, IHC, FACS, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibodyHSPD1 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 316-573) antibodyHSPD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, IEM 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) (AA 1-573) antibodyHSPD1 适用: 人 WB, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1) antibodyHSPD1 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Hamster, 犬, Cow, 兔, Pig, 绵羊, Drosophila melanogaster, 非洲爪蟾 WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal LK1 unconjugated
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应用备注
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Approved: IHC, IHC-P (10 μg/mL), IP (10 μg/mL), WB (0.25 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.2, 0.1 mM PMSF, 50 % glycerol
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储存液
- PMSF
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注意事项
- avoid freeze thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. For maximum product recovery, after thawing, centrifuge the product vial before removing cap.
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- HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))
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别名
- HSPD1 / HSP60
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背景
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Name/Gene ID: HSPD1
Synonyms: HSPD1, 60 kDa chaperonin, CPN60, Heat shock protein 65, Heat shock protein 60, HSP-60, HSP60, HuCHA60, HLD4, HSP65, p60 lymphocyte protein, SPG13, Chaperonin 60, GROEL -
基因ID
- 3329
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UniProt
- P10809
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途径
- Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
抗原
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