alpha 2 Macroglobulin 抗体
Quick Overview for alpha 2 Macroglobulin 抗体 (ABIN458423)
抗原
See all alpha 2 Macroglobulin (A2M) 抗体适用
宿主
克隆类型
标记
应用范围
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特异性
- Inter-species cross reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. of this antiserum has been tested in double radial immunodiffusion (Ouchterlony) with the following results: bovine ± cat ± chicken - dog - ferret ± goat - guinea pig ± hamster + horse ± human - monkey - pigeon - rat ++ sheep - swine ± turkey -
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产品特性
- Precipitating polyclonal rabbit antiserum to mouse alpha-2 macroglobulin
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纯化方法
- Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
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免疫原
- Alpha-2 macroglobulin ids the major protein in the alpha-2 region of mouse serum after separation by electrophoresis. It is a large polymer and a strong antigen. It forms about one-third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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亚型
- IgG
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anti-alpha-2-Macroglobulin (A2M) (AA 616-856) antibody
A2M 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 616-856) antibodyA2M 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C6 unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1053-1214) antibodyA2M 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1216-1467) antibodyA2M 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 1-1474) antibodyA2M 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) (AA 641-730) antibodyA2M 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2B5 unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, ELISA, IP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-alpha-2-Macroglobulin (A2M) antibodyA2M 适用: 人, Pig, 兔, 犬 IHC, ELISA, IP, IEP, IHC (p) 宿主: 山羊 Polyclonal unconjugated
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应用备注
- In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence alpha-2 macroglobulin in mouse serum or other body fluids and to determine its con-centration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.
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限制
- 仅限研究用
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状态
- Lyophilized
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浓度
- Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added. Antibody titre: Precipitin titre 1:32 when tested against pooled normal mouse sera of different strains in agar-block i
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缓冲液
- Delipidated, heat inactivated, lyophilized, stable whole antiserum
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储存液
- Without preservative
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储存条件
- 4 °C/-20 °C
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储存方法
- The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by add ing 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not ref rozen, and preferably used the same day.
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- alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))
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别名
- alpha-2 Macroglobulin
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背景
- The reactivity of the antiserum is restricted to alpha-2 macroglobulin as tested in immuno-electrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified alpha-2 macroglobulin
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途径
- Lipid Metabolism
抗原
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