Actin 抗体
(9 references)Quick Overview for Actin 抗体 (ABIN457424)
抗原
See all Actin (ACTA1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Actin (muscle) Purified
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特异性
- The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.
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交叉反应 （详细）
- Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken
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纯化方法
- Purified by protein-A affinity chromatography.
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纯度
- > 95 % (by SDS-PAGE)
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免疫原
- SDS extracted protein fraction of human myocardium
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亚型
- IgG1
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anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 277-308), (C-Term) antibody
ACTA1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人, 大鼠, 斑马鱼, 兔, Pig, 小鸡, 猴 WB, ELISA, IHC, IHC (p), IHC (fro), IEM 宿主: 小鼠 Monoclonal HHF35 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 3-377) antibodyACTA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 25E3 unconjugated Recombinant Antibody
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 1-129) antibodyACTA1 适用: 人, 斑马鱼, 鱼 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 346-375), (C-Term) antibodyACTA1 适用: 人 WB, IHC (p), IF, FACS 宿主: 兔 Polyclonal RB35391 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 人 WB, IHC (p), FACS 宿主: 小鼠 Monoclonal 7C1 unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) (AA 7-377) antibodyACTA1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 1, Skeletal Muscle (ACTA1) antibodyACTA1 适用: 小鸡 WB, IHC, IF 宿主: 小鼠 Monoclonal C4 unconjugated
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应用备注
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Western blotting: Recommended dilution: 1 μg/mL, positive control: murine femoral muscle, murine heart, negative control: HUVEC line, reducing conditions.
Immunohistochemistry (paraffin sections): Antigen retrieval steps generally not required, but e.g. in case of arterial smooth muscle cells or myoepithelial cells, pepsin or trypsin pretreatment is recommended. -
限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Do not freeze.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Do not freeze.
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: "Glomus tumor of the omentum: a case report." in: Journal of surgical oncology, Vol. 96, Issue 7, pp. 633-6, (2007) (PubMed).
: "Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma." in: Human pathology, Vol. 38, Issue 4, pp. 645-51, (2007) (PubMed).
: "Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver." in: Comparative hepatology, Vol. 5, pp. 7, (2006) (PubMed).
: "Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 5, pp. 547-52, (2001) (PubMed).
: "Basal cell adenocarcinoma of the palate with squamous metaplasia." in: Journal of clinical pathology, Vol. 53, Issue 2, pp. 153-6, (2000) (PubMed).
: "Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors." in: The American journal of pathology, Vol. 130, Issue 1, pp. 205-15, (1988) (PubMed).
: "Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins." in: The American journal of pathology, Vol. 131, Issue 1, pp. 19-28, (1988) (PubMed).
: "HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues." in: The American journal of pathology, Vol. 127, Issue 2, pp. 389-402, (1987) (PubMed).
: "HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization." in: The American journal of pathology, Vol. 126, Issue 1, pp. 51-60, (1987) (PubMed).
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: "Glomus tumor of the omentum: a case report." in: Journal of surgical oncology, Vol. 96, Issue 7, pp. 633-6, (2007) (PubMed).
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- Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
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别名
- Actin (muscle)
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背景
- Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.,Muscle specific actin
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途径
- Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
抗原
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