Western blotting demonstrated that this TGF-beta antibody reacts to either dimeric (25 kDa) or monomeric (12.5 kDa) molecules of TGF-beta under non-reducing and reducing condition respectively. Recognizes both Human platelet-derived and recombinant TGF beta in ELISA.
ELISA. Neutralizing Activity: This MAb neutralizes TGF beta activity in vitro in inhibition assay ofCCL/64 cell growth and also neutralize the growth promoting action of TGF-beta in theNRK-49F colony forming assays. The effect of micro-injection of this MAb into oneblastomere of two cell stage Xenopus embryos indicated that this MAb was able toneutralize effectively the bioactivity of TG beta in vivo. Immunohistochemistry: May be used in immunohistochemical techniques to locateTGF-beta within tissues. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with Double distillated water to adjust the final concentration to 1.00 mg/mL.
TGF beta 1 (Transforming Growth Factor Beta 1) is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non neoplastic tissues, release transforming growth factors, and essentially all cells possess a specific TGF beta 1 receptor. The multimodal nature of TGF beta 1 is seen in its ability to stimulate or inhibit cellular proliferation. TGF beta 1 plays a fundamental role in tissue growth and differentiation involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation and immune cell function. The C-terminal 112 amino acids constitute the mature protein.Synonyms: TGF-beta-1, TGFB, Transforming growth factor beta-1