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- 抗原 See all HSP27 (HSPB1) 抗体
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This HSP27 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects a 27 kDa protein corresponding to the Molecular Mass of hsp27 on SDS PAGE immunoblots. There is no cross-reactivity to alpha B-Crystallin.
- 交叉反应 （详细）
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Species reactivity (expected):Very limited crossreactivity to other species.
Species reactivity (tested):Human. - 产品特性
- Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein,SRP27, HSP25, Stress-responsive protein 27, Estrogen-regulated 24 kDa protein
- 纯化方法
- Protein G Chromatography
- 免疫原
- Human Hsp27
- 克隆位点
- 5D12-A12
- 亚型
- IgG2b
- Top Product
- Discover our top product HSPB1 Primary Antibody
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anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 适用: 人, 大鼠, 小鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, FACS, IP 宿主: 兔 Monoclonal 10C11 unconjugated Recombinant Antibody
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人, 大鼠, 小鼠, 绵羊, 猴, 小鸡, Chimpanzee WB, IHC, IF, FACS, ISt, StM 宿主: 小鼠 Monoclonal G3-1 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibodyHSPB1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 3G3 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibodyHSPB1 适用: 人, 大鼠, 小鼠 WB, IF, FACS, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, IF, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibodyHSPB1 适用: 人 WB, IHC, ELISA, IF, IP, ICC 宿主: 小鼠 Monoclonal 5D12-A12 unconjugated
anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody (HRP)HSPB1 适用: 人 WB, IHC, IF, FACS, IP, ICC 宿主: 小鼠 Monoclonal 8A7 HRP
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- 应用备注
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Western blot: 0.25 - 0.5 μg/mL. Immunoprecipitaion. ELISA. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- 别名
- HSPB1 / HSP27 (HSPB1 产品)
- 别名
- CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody
- 背景
- Hsp27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an α-crystallindomain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (6).Synonyms: 28 kDa heat shock protein, Estrogen-regulated 24 kDa protein, HSP25, HSP28, Heat shock 27 kDa protein, Heat shock protein beta-1, SRP27, Stress-responsive protein 27
- 基因ID
- 3315
- UniProt
- P04792
- 途径
- MAPK Pathway, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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