Reactive to serine133 phosphorylated Human CREB peptide fragment, and reactive to KLH or BSA conjugated Serine133 phosphorylated Human CREB peptide fragment. Not reactive to un-conjugated KLH or BSA.
交叉反应 (详细)
Species reactivity (tested):Human.
产品特性
Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsiveelement-binding protein 1
纯化方法
Affinity Chromatography on Protein G.
免疫原
Phosphorylated peptide fragment of CREB (pSer133), conjugated with KLH
Indirect ELISA: The suggested antigen coating quantity is 1 μg/mL of peptide/or peptideconjugating in pH 9.5 Carbonate buffer, 100 μL/well. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with Double distillated water to adjust the final concentration to 1.0 mg/mL.
缓冲液
0.01 M PBS, pH 7.0 without preservatives.
储存液
Without preservative
储存条件
-20 °C
储存方法
Store the antibody at -20 °C. After reconstitution, aliquot and store at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
有效期
12 months
抗原
CREB1
(cAMP Responsive Element Binding Protein 1 (CREB1))
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背景
Transcription factor CREB binds the cAMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity. Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1. CREB appears to play an important role in learning and memory in both flies and mice.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1