GRP94 抗体
Quick Overview for GRP94 抗体 (ABIN452391)
抗原
See all GRP94 (HSP90B1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody detects a 98 kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74 or GrpE from E. coli.
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交叉反应 （详细）
- Species reactivity (tested):Human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, horse, monkey, pig, rabbit, sheep, Xenopus
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产品特性
- Synonyms: Tumor rejection antigen 1, GRP94, Heat shock protein 90 kDa beta member 1, 94 kDaglucose-regulated protein
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纯化方法
- Protein G affinity chromatography
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免疫原
- Purified Grp94 isolated from chicken oviducts
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亚型
- IgG2a
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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibody
HSP90B1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Center) antibodyHSP90B1 适用: 人, 小鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibodyVerified HSP90B1 适用: 人, 小鼠 WB, IHC, FACS, IF, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibodyHSP90B1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, 小鸡, Cow, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 IHC, FACS, StM 宿主: 兔 Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, 小鸡, Cow, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 WB, IHC, FACS, IF, StM 宿主: 大鼠 Monoclonal 9G10-F8-2 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 687-700), (Middle Region) antibodyHSP90B1 适用: 人, 大鼠 WB, IHC, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 27-799) antibodyHSP90B1 适用: 人 WB, IHC, IF, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot (8, 9, 10). Immunoprecipitation (11-14). Flow cytometry.
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS pH 7.2, 50 % glycerol, 0.09 % azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C/-20 °C
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储存方法
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Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch. -
有效期
- 12 months
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- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
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别名
- Endoplasmin / HSP90B1 / TRA1
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背景
- Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90 (3). Both Hsp90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1
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基因ID
- 7184
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UniProt
- P14625
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途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
抗原
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